雾化吸入低分子量肝素对慢性阻塞性肺疾病大鼠血栓前状态干预的实验研究  

Effects of inhaled low molecular weight heparin on prethrombotic state in rat model of chronic obstructive pulmonary disease

作　　者：孙妮娜[1] 韩伟忠[1] 佟丽[1] 程兆忠[1] 

机构地区：[1]青岛大学医学院附属医院呼吸内科,266003

出　　处：《国际呼吸杂志》2008年第14期845-847,共3页International Journal of Respiration

摘　　要：目的探讨早期雾化吸入低分子量肝素（low molecular weight heparin，LMWH）对慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）大鼠模型发生发展的预防保护作用。方法采用香烟熏吸加气道内注入脂多糖法建立大鼠COPD模型，并用LMWH雾化吸入，观察支气管肺泡灌洗液（BALF）的细胞计数及分类、动脉血气、有关血液学指标、肺组织病理学改变。结果本实验建立被动吸烟致肺损伤大鼠模型的肺组织病理学改变基本符合人类COPD组织病理学改变特点，应用LMWH后，BALF中白细胞总数及巨噬细胞数较模型组显著降低，血液中vWF因子含量明显减低，PaO2、PaCO2明显改善。结论早期雾化吸入LMWH对大鼠COPD模型的发生发展有一定的预防保护作用。Objective To investigate the activity of coagulation and fibrinolysis in blood, and to observe the precautionary effect of inhaled low molecular weight heparin （LMWH） on rat of chronic obstructive pulmonary disease（COPD）. Methods The rat of COPD model （model group） was established by intratracheal instillation of lipopolysaccharide （LPS） twice and exposured to cigarette smoke, meanwhile given LMWH inhaled. The activity of coagulation and fibrinolysis in blood, the blood gas analysis, the pathologic characteristics of bronchial walls,the total and different white blood cell counts of bronchoalveolar lavage fluid were determined. Results The model group rats shared specific pathological features in trachea, bronchi and lung tissues with that of human chronic bronchitis and obstructive emphesema; significant increase of total white blood cells and neutrophils in BALF was found in COPD group compared with those of control group （ P 〈 0.05）. The total white blood cells and neutrophils in BALF in precaution group decreased obviously than in COPD group（ P 〈0.05）, and the ratio of monocyte-macrophage almost returned to the normal;vWF in blood significantly decreased in precaution group than in COPD group（ P 〈0.05）; PaO2 ,PaCO2 improved significantly（ P 〈0.05）. Conclusions There is preventive and protective effect of LMWH inhaled in the early state of COPD in some extent.

关 键 词：低分子量肝素 慢性阻塞性肺疾病 血管内皮 

分 类 号：R563[医药卫生—呼吸系统] R973.2[医药卫生—内科学]