大黄总蒽醌对大鼠远端结肠AQP2表达的调节效应  

Effect of total anthraquinone in rheum on aquaporin 2 expression in rat distal colon

作　　者：鲍军强[1] 李锋[1] 张文生[1] 徐彦博[1] 刘青[1] 王新[2] 赵一岭[3] 王长海[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032 [2]第四军医大学西京医院消化内科,陕西西安710032 [3]第四军医大学病理学教研室,陕西西安710032

出　　处：《中国中药杂志》2008年第14期1732-1735,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572385)

摘　　要：目的:探讨大黄总蒽醌对大鼠远端结肠水通道蛋白2(aquaporin2,AQP2)表达的调节作用。方法:32只SD大鼠随机分为正常组,大黄总蒽醌低、中、高剂量组(0.14,2.5,4.5 g·kg^(-1)·d^(-1),灌胃),正常组给予生理盐水灌胃。大鼠5 d后麻醉处死,取全段结肠内粪便,干燥后检测粪便含水量;留取远端结肠,运用免疫组织化学、Western Not及RT-PCR检测远端结肠AQP2表达的变化。结果:低剂量组大鼠没有出现泻下现象,其远端结肠AQP2表达无显著差异;中剂量组(2.5 g·kg^(-1)·d^(-1))大鼠出现软便及稀便,高剂量组(4.5 g·kg^(-1)·d^(-1))大鼠出现稀便和水样便,粪便含水量显著性增加,其远端结肠AQP2表达亦显著降低(P<0.01)。结论:大黄总蒽醌能抑制大鼠远端结肠AQP2的转录与翻译、增加粪便的含水量,这可能是其泻下效应产生的机制之一。Objective： To investigate effect of total anthraquinone in rheum on aquaporin 2 expression in rat＇ distal colon. Method： SD rats were randomly divided into control group, low dose group, middle dose group and high dose group. Gavaged to control group, and treated group were administered saline and total anthraquinone in rheum with dosage of 0. 14, 2. 5, 4. 5 g·kg^-1·d^-1, respectively. All rats were put sacrificed after 5 days and stool in full length colon was gently collected to detect water content stool. Distal colon was removed to detect AQP2 expression with immunohistochemistry, western blot and RT-PCR. Result： No diarrhea was found in low dose group and control group, there were not significant difference water content of stool and AQP2 expression between low dose group and control group. However, soft feces and loose stools occurred in diarrheic dose group, loose stools and watery stool appeared in high dose group. Stool water content increased in diarrheic dose group and High dose group, expression of AQP2 decreased evidently in these two groups （P 〈 0.01 ）. Conclusion： Total anthraquinone in rheum can reduce the transcription and translation of AQP2 in rats＇distal colon, increase fecal water content, which probably is one of the mechanisms of diarrhea caused by total anthraquinone in rheum.

关 键 词：大黄总蒽醌 大鼠 泻下作用 远端结肠 水通道蛋白2 

分 类 号：R285.5[医药卫生—中药学]