平郁胶囊对慢性应激抑郁大鼠受体后信号传导通路的影响  

Effects of Pingyu capsule on signal transduction of rats with chronic stress-induced depression

作　　者：吴立坤[1] 张建军[1] 王景霞[1] 李伟[1] 邓晓迎[1] 佟海英[1] 周鹏[1] 奚胜艳[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中药杂志》2008年第14期1743-1746,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察平郁胶囊对慢性应激抑郁大鼠受体后信号传导通路重要调节因子cAMP,PKA,PKC的影响。方法:Wistar雄性大鼠,随机分为空白组,模型组,平郁胶囊低、中、高剂量组,阳性药帕罗西汀组。除空白组外其余各组大鼠均单笼孤养,并且给予电击足底、冰水游泳、热烘、夹尾等不可预见刺激,周期为21 d。取出大脑的海马和皮质,用放射性免疫法检测其cAMP的含量,用Elisa法检测其PKA和PKC的含量,用放射性同位素法检测其PKA和PKC的活性。结果:模型组大鼠海马和皮质内的cAMP含量、海马内PKA含量及活性、皮质内PKC含量均显著低于正常组,平郁胶囊及阳性药帕罗西汀均能使之上调。结论:平郁胶囊可能是通过调节受体后cAMP-PKA信号传导通路而发挥抗抑郁作用。Objective： To observe the effects of Pingyu capsule on such important regulatory factors as cAMP, PKA and PKC of the signal transduction in rats with chronic stress-induced depression. Method： Wistar male rats were randomly divided into control group, model group, paroxetine group, and Pingyu capsule low, middle and high doses groups; rats in all groups but the control group were fed in single cage for 21 days and were given such irritations as lightening stroke on pelma, ice water swimming, pyretic fumiga- tion and tail clipping during this period; lobe cortex and hippocampus of all rats were taken out for detection of the cAMP content by means of radioactive immunization, PKA and PKC content by Elisa, and the PKA and PKC activity by radioactive isotope. Result： Content of cAMP in lobe cortex and hippocampus, content and activity of PKA in hippocampus, and content of PKC in lobe cortex of rats in the model group were lower than those in the normal group. Pingyu capsule and paroxetine can increase them. Conclusion： The anti-depression effect of Pingyu capsule maybe related to its function of adjusting the signal transduction of cAMP-PKA.

关 键 词：平郁胶囊 抑郁症 慢性应激 cAMP PKA PKC 800205a 

分 类 号：R285.5[医药卫生—中药学]