盾叶薯蓣原生质体分离  

Protoplast Isolation in Dioscorea zingibernsis Wright

作　　者：刘艳丽[1] 姚家玲[2] 

机构地区：[1]武汉科技大学中南分校生命科学学院,武汉430223 [2]华中农业大学生命科学技术学院,武汉430070

出　　处：《华中农业大学学报》2008年第3期430-433,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(39770084)资助

摘　　要：以盾叶薯蓣叶片、种子愈伤为材料,探讨了不同的酶浓度、酶解时间及渗透压对原生质体分离的影响。结果表明：生长5～10 d的叶片置于0.6%纤维素酶、0.5%果胶酶、0.9 mol/L甘露醇的酶液中、黑暗处理21 h,其原生质体产量和活力最高;悬浮培养5～10 d的愈伤组织在2.0%纤维素酶、1.0%果胶酶、0.7 mol/L甘露醇的混合酶液中黑暗处理6～8 h原生质体产量和活力最高。Young leaves,and calli were excised as material,and the effect of different enzyme combination, time of enzymolysis, osmotic pressure on protoplast isolation were studied. The results are as follow：Leaves which have grown for 5-10 days are cut up, then were put into enzyme which consists of 0. 6% cellulase,0. 5% pectinase and 0. 9 mol/L mannitol and are sustaining for 21 hours in dark condition,its protoplast yield and activity is the highest; Calli which have suspended for 5 days are put into enzyme including 2. 0% cellulase, 1.0% pectinase and 0. 7 mol/L mannitol for 6-8 hours in dark condition,its protoplast yield and activity is the best.

关 键 词：盾叶薯蓣 原生质体 分离 

分 类 号：S567.203.53[农业科学—中草药栽培]