白首乌甾苷元C11α-羟基化的两种微生物转化  

C11α-Hydroxylation of C_(21) Steroidal Aglycones in Cynanchum Auriculatum by Transformation with Two Kinds of Microbes

作　　者：李于善[1] 贺艳[1] 

机构地区：[1]三峡大学天然产物研究与应用湖北省重点实验室,湖北宜昌443002

出　　处：《化学与生物工程》2008年第7期48-50,共3页Chemistry & Bioengineering

摘　　要：以自三峡白首乌提取分离得到的C21甾苷元告达庭甾苷元和开德甾苷元作为底物,采用黑根霉（Rhizopus nigricans）和赭曲霉（Aspergillus ochraceus.）两种微生物在水-正丁醇双相体系中,以连续转化或同步转化的方法制备C11α-羟基化的白首乌C21甾苷元告达庭甾苷元和开德甾苷元,羟化反应控制pH值在5.0-6.0,于30℃下振荡培养70 h。产物经红外、紫外、质谱、核磁共振图谱分析表征。结果表明,同步转化法相对较好,其副产物少,收率达74.1%。Using C21 steroidal aglycones（Caudatin steroidal aglycones and Kaider steroidal aglycones） extracted from Sanxia Cynanchum auriculatum as substrate, C11α-hydroxylated Cynanchum auriculatum C21 steroidal aglycones were prepared by Rhizopus nigricans and Aspergillus ochraceus, continuously or synchronally in water-n-butanol bipolar system. During the microbiological transformation process, the pH value was kept at 5.0-6.0, the medium were shaking cultivated for 70 hours at 30℃, and the products were determined as C11α-hydroxylated Caudatin steroidal aglycones and C11α-hydroxylated Kaider steroidal aglycones by infrared spectroscopy,UV spectroscopy, mass spectroscopy, and nuclear magnetic resonance spectroscopy. The results showed that the synchronized transformation method was relatively better with less side reactions and high yield of 74.1%.

关 键 词：黑根霉 赭曲霉 C11α-羟基化 C21甾苷元 

分 类 号：TQ463.24[化学工程—制药化工] TQ460.38