4种异黄酮改构化合物对人离体子宫内膜腺上皮细胞增殖活性的比较  

Biological effect of four isoflavone derivatives on proliferation of endometrial epithelial cells in vitro

作　　者：陈诚[1] 常青[1] 张丽龙[2] 艾国平[2] 

机构地区：[1]第三军医大学西南医院妇产科 [2]第三军医大学预防医学院防原医学教研室,全军复合研究所,烧伤,创伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2008年第16期1553-1556,共4页Journal of Third Military Medical University

基　　金：重庆市科技攻关项目(2004BB5034)~~

摘　　要：目的观察4种异黄酮改构化合物(F8、F11、ZF3和ZF7)对人离体子宫内膜腺上皮细胞增殖的影响。方法采用胶原酶消化+二次筛网过滤法原代培养人子宫内膜腺上皮细胞。MTT法检测并比较4种化合物对人离体子宫内膜腺上皮细胞增殖的影响。结果成功培养原代人离体子宫内膜腺上皮细胞并稳定传代。4种异黄酮化合物作用于人子宫内膜腺上皮细胞24h后,低剂量(25μmol/L)F8显著促进细胞增殖(P<0.05),中高剂量(50、100μmol/L)F11显著抑制细胞增殖(P<0.05);作用48h后,中剂量(50μmol/L)F11和ZF3显著抑制细胞增殖(P<0.05),高剂量(100μmol/L)化合物均显著抑制细胞增殖(P<0.05);作用72h后,除低剂量(25μmol/L)F8、ZF3和ZF7未明显抑制细胞增殖外(P>0.05),各化合物在不同剂量下均抑制细胞增殖效应(P<0.05);该生物学效应具有时间和剂量依赖性。结论4种异黄酮化合物在一定剂量下作用一段时间后可抑制人离体子宫内膜腺上皮增殖。F11抑制细胞增殖活性最强。Objective To study the biological effects of four isoflavone derivatives （F8, F11, ZF3 and ZFT） on endometrial epithelial cells. Methods The endometrial epithelial cells were cultured through collagenase enzymatic digestion and twice grit filtration. The biological effects on endometrial epithelial cells of four isoflavone derivatives were compared through MTT. Results The primary endometrial epithelial cells were successfully disassociated, cultured and passaged down stably. Cell proliferation was significantly increased by F8 （25 mol/L） （P 〈0.05）, but significantly inhibited by F11 （50 and 100 mol/L） （P 〈 0.05 ） 24 h after four isoflavone derivatives acting on endometrial epithelial cells. Cell proliferation was significantly inhibited by Fll, ZF3, ZF7 （50 mol/L） （P 〈0. 05）and all the 4 isoflavone derivatives （100 mol/L） 48 h later. Cell pro- liferation, not inhibited by F8, ZF3 and ZF7 （25 tool/L） , can be inhibited by four isoflavone derivatives of other dosage （P 〈0. 05） 72 h later. Their biological effects are time- and dose- dependent. Conclusion All the 4 isoflavone derivatives can inhibit the cell proliferation in vitro, among which F11 has the strongest effect.

关 键 词：异黄酮改构化合物 子宫内膜腺上皮细胞 细胞增殖 

分 类 号：R322.65[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]