饲料蛋白水平对异育银鲫雌性性腺发育的影响  

EFFECT OF DIETARY PROTEIN LEVEL ON THE GONAD DEVELOPMENT OF FEMALE ALLOGYNOGENETIC CRUCIAN CARP

作　　者：龙勇[1] 李芹[1] 罗莉[1] 刁晓明[1] 

机构地区：[1]西南大学动物科技学院水产系

出　　处：《水生生物学报》2008年第4期551-557,共7页Acta Hydrobiologica Sinica

基　　金：重庆市科委应用基金(6362)资助

摘　　要：采用鱼粉、玉米淀粉、羧甲基纤维素、菜油、Ca(H2PO4)2和维生素矿物质复合预混料等配制蛋白含量分别为0%、16%、24%、32%和40%的5种等能饲料,并设置一饥饿组,研究了饲料蛋白水平对异育银鲫雌性性腺发育的影响。试验鱼为同一批亲鱼繁殖的当年鱼种,平均体重(30.0±5.0)g。试验在室内循环水养殖系统中进行,每天照明12h(8:00—20:00),每组各设4个重复,总共进行20周的人工养殖试验。每月中旬取样检测血清卵黄蛋白原磷含量和性腺系数,试验结束时分析肌肉和性腺的生化组成,测量繁殖力和成熟卵的卵径。试验结果表明:从11月到翌年2月,除饥饿组血清卵黄蛋白原磷含量一直降低外,其余各组随饲料蛋白水平的升高而升高;到3月份,随着性腺发育成熟,各组的血清卵黄蛋白原磷含量都急剧降低。从11月份到1月份,饲料蛋白水平对性腺系数的影响不明显;2月份和3月份,饲料蛋白水平对性腺系数产生了显著影响,性腺系数随饲料蛋白水平的升高而增大。肌肉、性腺的蛋白质和脂肪含量随饲料蛋白水平的升高而升高,水分含量随饲料蛋白水平的升高而降低。饥饿组和0%组的繁殖力显著低于其他各组,16%、24%和32%组之间无显著差异,40%组显著高于其他各组。饥饿组的卵径显著低于其他各组,饲料蛋白水平为0%—32%的各组之间无显著差异,40%组的卵径最大,显著高于饥饿组和0%组。Five isocaloric diets containing 0% , 16% , 24% ,32% and 40% crude protein respectively were formulated by using fish meal, corn starch, CM-cellulose, rape oil, Ca （ H2PO4 ） 2, vitamin and mineral premixture, concomitantly, a starvation group was set to study the effect of dietary protein level on the gonad development of female Allogynogenetic crucian carp. Fishes from the same brood stock and with an average body weight of （30.0 ± 5.0） g were randomly stocked in quadruple groups and raised in an indoor flow-through system with a light regime of 12h： 12h（ 8：00--20：00）. The experiment lasted for 20 weeks from November to March of the next year. Samples were taken at the mid of each month to determine the serum vitellogenin content and gonad somatic index. At the end of the trial, the biochemical composition of muscle and gonad were analyzed and the fecundity of fish and the diameter of mature eggs were measured. The results indicated that from November to February,except for the serum vitellogenin content of the starvation group kept declining,the content in the other groups increased with the rise of dietary protein level. But in March, because of the maturation of ovary, the serum vitellogenin content in all groups declined sharply. Although dietary protein level had no significant effect on the gonad somatic index before January, the gonad somatic index was significantly influenced by dietary protein level in February and March, which increased with the rise of dietary protein level. Along with the increase of dietary protein level, the muscle and gonad＇s protein and lipid content ascended and the moisture content descended significantly. The starvation group had the lowest fecundity and the 40% group had the highest value, but there was no significant difference among the groups of 16% ,24% and 32%. The egg＇s diameter of the starvation group was remarkably lower than other group. The 40% group had the highest value which significantly higher than the starvation group and the 0% group. B

关 键 词：蛋白水平 异育银鲫 性腺发育 繁殖力 

分 类 号：S963.1[农业科学—水产养殖]