大鼠自体肝移植中三种不同肝脏灌注方法的比较  

Comparison of three different liver’s perfusions in liver transplantation of rats

作　　者：苏树炎[1] 霍枫[1] 

机构地区：[1]广州军区广州总医院肝胆外科,广州510010

出　　处：《肝胆胰外科杂志》2008年第4期248-250,253,共4页Journal of Hepatopancreatobiliary Surgery

基　　金：广州市科技攻关基金项目(2006Z3-D0381)

摘　　要：目的比较三种不同的肝脏冷灌注方法的灌注效果。方法建立大鼠自体肝移植模型60例,通过不同的冷灌注方法(A经门静脉灌注法、B经腹主动脉灌注法、C经门静脉和腹主动脉同时灌注法),比较其灌注效果、术后存活情况和手术时间。结果A法灌注后光镜下可见小叶间动脉红细胞残存;B法、C法灌注后小叶间动脉、静脉均未见红细胞残留。各组手术时间分别为(50±3.0)min,(53±4.0)min,(58±3.5)min;术后存活率分别为95%(19/20),90%(18/20),80%(16/20)。结论B法是三种不同冷灌注方法中相对既简单又彻底的一种,对大鼠自体(或异体)肝移植冷灌注可优先选用;但对于非吻合肝动脉及与肝动脉无关的研究也可选用A法灌注;而C法并非必需。Objective To study the result of three different cold perfusing liver methods in rat. Methods The model orthotopic liver autotransplatation in rat was set up. Sixty SD rats were randomly divided into groups A, B, C; the processing method that it was perfused via portal vein, via abdominal aorta and simultaneous via portal vein and abdominal aorta respectively. In the three groups, the result of the liver after perfusion, the operation time and the survival rate were compared. Results There was erythrocytes in the interlobular arteries under the light microscope in group A, and did not find erythrocyte in the interlobular arteries and interlobular veins in group B and group C. The operation time of group A, B, C were （50±3.0） rain, （53±4.0） rain and （58±3.5） rain, respectively; the survival rate was 95%（19/20）, 90%（18/20） and 80% （16/20） respectively. Conclusion The group B correspondingly is the simple and thorough method in the three methods, can be first chosen for rat liver transplantation; but non-hepatic artery anastomosis or the research nothing to do on the hepatic artery may also choose group A, but the group C is not necessary.

关 键 词：冷灌注 方法 效果 自体肝移植 大鼠 模型 

分 类 号：R657.3[医药卫生—外科学]