噪声对生长发育期大鼠学习记忆行为及GABA神经元表达的影响  

The influence of noise on learning memory behaviors in and expression of GABA neurons in hippocampus

作　　者：汤艳[1] 程淑群[1] 袁海艳[1] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生学教研室

出　　处：《重庆医科大学学报》2008年第5期557-559,587,共4页Journal of Chongqing Medical University

基　　金：重庆市教育委员会资助(99022)

摘　　要：目的:探讨噪声对生长发育期大鼠学习记忆能力的影响及其机制。方法:将24只Wistar幼鼠,随机分为噪声组和对照组,噪声组在90dB(A)噪声下持续暴露1个月,用Morris水迷宫测定大鼠的空间学习记忆能力,用免疫组化法观察其海马区r-氨基丁酸(Aminobutyric acid,GABA)阳性神经元的表达。结果:在Morris水迷宫试验中,噪声组寻找平台的潜伏期较对照组延长(P<0.05),末次跨台次数较对照组减少(P<0.01)。噪声组海马区GABA阳性神经元的数量及染色强度较对照组显著降低。结论:噪声可使生长发育期大鼠学习记忆能力下降,这可能与海马区GABA的合成减少,使神经元的突触抑制效应减弱,LTP的诱导和维持受损有关。Objective：To probe the effect of noise on the capabilities of learning and memory of growing rat and its possible mechanism. Methods：24 Wistar growing rats were divided randomly into two groups, noise group and control group. The growing rats in the noise group were exposed to 90dB（ A ） noise constantly for one month. The capabilities of learning and memory of growing rats were measured by means of Morris water maze test. An immunohistochemical method was used to observe the expression of aminobuty-ric acid（GABA） positive neurons in hippocampus. Results：The test of Morris water maze showed that the mean escape latency of growing rats in the noise group were longer than that in the control group and the frequency to bestride platform were less. The number of GABA positive neurons and the magnitude of tinction in hippocampus of growing rats in the noise group were reduced markedly than that in the control group. Conclusion：The noise does impair the capabilities of learning and memory of growing rats. The impairment may be related with the reduction of GABA contents in hippocampus, which could decrease the depressive effect of neuronic synapse and impair the induction and maintenance of the long term potentiation.

关 键 词：噪声 学习记忆 海马 GABA 

分 类 号：R134.1[医药卫生—劳动卫生]