3种不同方法检测甲胎蛋白的比较  

Determination of serumα-fetoprotein by TRFIA,RIA and ELISA

作　　者：陈海蔚[1] 伍勇[1] 

机构地区：[1]中南大学湘雅三医院检验科,长沙410003

出　　处：《重庆医科大学学报》2008年第5期629-630,640,共3页Journal of Chongqing Medical University

摘　　要：目的:比较测定甲胎蛋白(Alpha-fetoprotein,AFP)的3种不同方法。方法:采用时间分辨荧光免疫分析法(Time-re- solved fluoroimmuno assay,TRFIA)、放射免疫法(Radio immuno assay,RIA)与酶联免疫吸附法(Enzme linked immunoabsorbent assay,ELISA)。分别进行线性、精密度、对比实验比较。结果:3种方法中,TRFIA法线性范围最宽;精密度试验中TRFIA法批内为3.3%,批间为4.7%;RIA法批内为5.3%,批间为8.9%;ELISA法批内为10.5%,批间为15.2%。比较试验显示RIA法与TRFIA法的结果相关性比ELISA法与RIA法好。结论:TRFIA法测定AFP优于RIA法和ELISA法。Objective：To assess the efficacy of time-resolved fluoroimmuno assay（TRFIA） for quantative determination of α-fetoprotein（AFP） in comparison with radio immuno assay（ RIA ） and enzme linked immunoabsorbent assay（ ELISA ）. Methods：Thirty serum samples from randomly selected patients were collected to test AFP content with TRFIA,RIA and ELISA respectively. Results：The linear range of AFP was 1 to 1 000 ng/ml for TRFIA, 5 -400ng/ml for RIA and 5-300ng/ml for ELISA. The intraassay coefficients of variation（CV） and interassay CV were 3.3% and 4.7% for TRFIA,5.3% and 8.9% for RIA,and 10.5% and 15.2% for ELISA,respectively. Correlation tests revealed that the results of RIA were more closely correlated with those of TRFIA than with those of ELISA. Conclusion：TRFIA is shown to be a better method for AFP quantitation than RIA and ELISA in terms of precision and linear range.

关 键 词：甲胎蛋白 时间分辨荧光免疫分析法 放射免疫法 酶联免疫吸附法 

分 类 号：R446.61[医药卫生—诊断学]