新生大鼠海马神经干细胞表达趋化因子受体CX3CR1的体外研究  

In vitro study on expression of chemokine receptor CX3CR1 in neural stem cells of neonatal rat hippocampi

作　　者：丁鹏[1] 薛黎萍[2] 王嘉沪[1] 宋晓斌[1] 李智高[1] 蔡山[1] 冯曜宇[1] 冯忠堂[3] 

机构地区：[1]昆明医学院第一附属医院神经外科,昆明650032 [2]云南省第二人民医院眼科,昆明650021 [3]昆明医学院神经科学研究所,昆明650031

出　　处：《中华神经医学杂志》2008年第7期681-683,共3页Chinese Journal of Neuromedicine

摘　　要：目的探讨体外培养神经干细胞是否表达趋化因子受体CX3CR1。方法采用无血清方法分离、培养新生大鼠海马神经干细胞，细胞免疫荧光方法检测神经干细胞标志巢蛋白（nestin）表达及干细胞多向分化为神经元及胶质细胞的能力，后通过细胞免疫荧光及RT-PCR方法检测神经干细胞表达趋化因子受体CX3CR1的情况。结果体外培养新生大鼠海马神经干细胞呈nestin阳性，可分化为神经丝蛋白200（NF200）阳性的神经元、胶质纤维酸性蛋白（GFAP）阳性的星形胶质细胞及2^＋，3^＋．环核苷酸-3^＋-磷酸二酯酶（CNP）阳性的少突胶质细胞，细胞免疫荧光及RT-PCR证实神经干细胞表达趋化因子受体CX3CR1。结论新生大鼠海马神经干细胞表达趋化因子受体CX3CR1，为进一步研究其体内外迁移提供理论依据。Objective To explore whether neural stem cells in vitro express the chemokine receptor CX3CR1. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in a serum-free medium. Using immunofluorescence, the neural stem cells and their differentiation into neural and glial cell types were identified; then, the immunofluorescence and RT-PCR were employed to detect whether the neural stem cells in vitro expressed chemokine receptor CX3CR1. Results The floating neural spheres, isolated from neonatal rat hippocampi, were nestin positive and could differentiate into cells to express characteristic antigens of neurons, astrocytes and oligodendrocytes. Immunofluorescence and RT-PCR showed that neural stem cells expressed chemokine receptor CX3CR1. Conclusion Neural stem cells in vitro can express chemokine receptor CX3CR1, which offers the theoretical evidence for further study of its migration.

关 键 词：神经干细胞 趋化因子受体 CX3CR1 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]