创伤性脑损伤后大鼠脑组织CtyC与AIF的变化及其对神经细胞的影响  

Changes of CtyC and AIF in brain and effects of them on neuronal cells in rats after traumatic brain injury

作　　者：瞿文军[1] 徐如祥[2] 蔡颖谦[2] 黄东健[3] 

机构地区：[1]广东省第二人民医院神经外科,广州510317 [2]南方医科大学珠江医院神经外科,广州510282 [3]广州医学院第三附属医院ICUI,广州510150

出　　处：《中华神经医学杂志》2008年第7期691-694,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨创伤性脑损伤后大鼠脑组织中细胞色素C（CtyC）与凋亡诱导因子（AIF）的变化及其对神经细胞的影响。方法70只SD大鼠按随机数字表法分为假损伤组（n=10）和脑损伤组，脑损伤组按伤后1、6、24、48、72和168h观察时间点分为6个亚组（n=10）。用Feeny氏自由落体撞击法制作重型颅脑损伤模型。利用免疫荧光和共聚焦显微镜技术测定损伤后不同时间点CtyC和AIF的荧光表达强度，用免疫荧光双标记技术观察损伤后24h时CtyC及AIF对神经细胞的影响。结果（1）脑损伤后脑组织中CtyC变化：伤后1、6、24、48h，皮层CtyC分别增高为1．89±0．03、2．69±0．07、2．99±0．06、3．05±0．05，海马CtyC分别增高为1．34±0．04、1．87±0．03、2．60±0．03、2．80±0．06，明显高于假损伤组．差异有统计学意义fP〈0．05）。72h皮层与海马CtyC分别降为1．94±0．05及1．12±0．04，亦明显高于假损伤组，差异有统计学意义（P〈0．05）。168h皮层与海马CtyC与假损伤组比较差异无统计学意义（P〉0．05）。（2）脑损伤后脑组织中AIF变化：伤后1、6、24、48h，皮层AIF分别增高为1．82±0．16、2．16±0．34、2．75±0．22、2．87±0．12，海马AIF分别增高为1．27±0．06、2．01±0．05、2．49±0．02、2．62±0．05，明显高于假损伤组，差异有统计学意义（P〈0．05）。72h皮层与海马AIF分别降为1．35±0．09及1．32±0．05，亦明显高于假损伤组，差异有统计学意义（P〈0．05）。168h皮层与海马AIF恢复正常水平，与假损伤组比较差异无统计学意义（P〉0．05）。（3）CtyC与AIF都能引起神经细胞凋亡。结论创伤性脑损伤后CtyC和AIF可从线粒体释放，并起到凋亡因子的作用．诱导神经细胞发生凋亡。Objective To study the changes ofcytochrome C （CtyC） and apoptosis-induced factor （AIF） in the brain and effects of them on neuronal cells in rats after traumatic brain injury （TBI）. Methods Seventy Sprague-Dawley （SD） rats were randomly divided into sham-injury group （n=10） and brain injury group. The brain injury group was divided into 6 subgroups according to 1, 6, 24, 48, 72, 168 h after traumatic brain injury （n=10）. Rat models with contusion and laceration of brain were established by Feeney＇s impact with free falling. Fluorescence intensity of CtyC and AIF were observed by immunofluorescence and confocal laser scanning microscopy at different time points. The effects of CtyC and AIF on neuronal cells were observed by double-labeled immunofluorescence. Results （1）The changes of CtyC in the brain after TBI： at 1, 6, 24 and 48 h following TBI, CtyC was 1.89±0.03, 2.69±0.07, 2.99±0.06 and 3.05±0.05 in the cortex and 1.34±0.04, 1.87±0.03, 2.60±0.03 and 2.80±0.06 in the hippocampus, respectively, being significantly higher than that of sham-injury group （P〈0.05）; at 72 h, CtyC declined to 1.94±0.05 in the cortex and to 1.12±0.04 in the hippocampus, respectively, being significantly higher than that of sham-injury group （P〈0.05）; at 168 h, CtyC recovered to the normal level in the cortex and hippocampus, being not significantly different between injury group and sham-injury group. （2） The changes of AIF in the brain after TBI： at 1, 6, 24 and 48 h following TBI, AIF was 1.82± 0.16, 2.16±0.34, 2.75±0.22, 2.87±0.12 in the cortex and 1.27±0.06, 2.01±0.05, 2.49±0.02, 2.62±0.05 in the hippocampus, respectively, being significantly higher than that of sham-injury group （P〈0.05）; at 72 h,AIF declined to 1.35 ＋0.09 in the cortex and to 1.32±0.05 in the hippocampus, respectively, being significantly higher than that of sham-injury group （P〈0.05）; at 168 h, AIF recovered to the normal level in the cortex and hippocampus, being not significan

关 键 词：颅脑损伤 细胞色素C类 凋亡诱导因子 细胞凋亡 

分 类 号：R651.15[医药卫生—外科学]