注射用内给氧对缺血再灌注损伤兔肝脏SOD、GSH-PX活性和MDA含量的影响  

Effects of Intra Oxygen Injection on SOD, GSH-PX and MDA Levels of Hepatic Ischemia Reperfusion Injury in Rabbits

作　　者：陈云华 陈卫东[2] 

机构地区：[1]湖南省建设医院,长沙410007 [2]中南大学湘雅二医院普通外科,长沙410011

出　　处：《热带医学杂志》2008年第7期654-656,共3页Journal of Tropical Medicine

摘　　要：目的探讨注射用内给氧对缺血再灌注(ischemia/reperfusion,I/R)损伤兔肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性和丙二醛(MDA)含量的影响。方法48只健康新西兰长耳大白兔随机分为四组:假手术组(A组)、缺血再灌注组(B组)、缺血再灌注+周围静脉注射用内给氧组(C组)、缺血再灌注+肝动脉注射内给氧组(D组),每组12只。采用Pringle氏法建立肝脏I/R模型。比较四组大白兔缺血再灌注后1、2、24h肝组织的抗氧化能力(SOD、GSH-PX)、脂质过氧化产物丙二酮(MDA)含量。结果与A组比较,B、C、D三组肝组织SOD、GSH-PX活力降低,MDA含量增高(P<0.05);与B组比较,C、D两组肝组织SOD、GSH-PX活力升高,MDA含量降低(P<0.05);C组与D组比较,各参数差异无统计学意义(P>0.05)。结论注射用内给氧对肝脏缺血再灌注损伤有保护作用,可降低MDA含量,提高SOD活力和GSH-PX活性。Objective To investigate the effects of intra oxygen injection on superoxide dismutase （SOD）, glutothine peroxidase （GSH-PX） and malondi-aldehyde （MDA） levels in hepatic ischemia/reperfusion injury. Methods 48 rabbits were randomized divided into four groups of A （sham surgery group）, B （ischemia/reperfusion group）, C （I/ R＋intra oxygen injection from peripheral vein group） and D （I/R＋intra oxygen injection from hepatic artery group）. Pringle＇s way was used to set up hepatic ischemia/reperfusion model. The anti-oxidation capacity of the liver tissue （SOD and GSH-PX） and the content of MDA were determined at 1, 2, and 24 hour after surgery in each group. Results The content of MDA in group B, C and D were higher than group A, while the activities of SOD and GSH-PX in the liver tissue were opposite, both of which showed significant differences. However, compared to group C and D, the activities of SOD and GSH-PX in the liver tissue were higher, while the content of MDA were lower. All the indexes in groups C and D showed no significant differences. Conclusion Intra oxygen injection can protect the liver from I/R injury, possibly by lowering the content of MDA, improving the activities of SOD and GSH-PX.

关 键 词：注射用内给氧 肝脏 缺血再灌注损伤 

分 类 号：R657.3[医药卫生—外科学]