穿心莲内酯体外抗白念珠菌生物膜作用的初步研究  

In vitro activity of andrographolide against Candida albicans biofilms

作　　者：汪长中[1] 程惠娟[1] 官妍[1] 汪斌[1] 洪静[1] 云云[1] 王艳[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,合肥230038

出　　处：《中国真菌学杂志》2008年第3期134-137,共4页Chinese Journal of Mycology

基　　金：安徽省高校自然科学研究项目(KJ2008B223);安徽省科技厅重点项目(07021003)

摘　　要：目的研究穿心莲内酯对体外白念珠菌生物膜的影响。方法采用XTT减低法评价穿心莲内酯对白念珠菌生物膜及其黏附性的影响;镜下观察该药对白念珠菌生物膜的形态学影响;细胞毒性试验检测该药的毒副作用。结果穿心莲内酯对白念珠菌生物膜的SMIC50、SMIC80分别是250、1000μg/ml;1000μg/ml及100μg/ml时对白念珠菌的早期黏附及菌丝生长有抑制作用;对人细胞毒性较弱。结论穿心莲内酯对体外白念珠菌生物膜有显著的抑制作用。Objective To investigate the in vitro effects of andrographolide on C. albicans biofilms. Methods XTT reduction assay was performed to determine the effect of andrographolide on C. albicans biofilms and adherence. Microscopic examination was conducted to assess the effect of andrographolide on morphogenesis of C. albicans biofilms. Cytotoxic assay was used to measure the ad- verse effects of andrographolide. Results SMIC50 and SMIC80 of andrographolide against C. albicans biofilms was 250 and 1 000 μg/ml, respectively. At the concentration of 1 000μg/ml and 100μg/ml, andrographolide could inhibit the initial adherence and filamentous growth, and the agent showed poor cytotoxic activity. Conclusion Andrographolide displays potent activity against C. albicans biofilm.

关 键 词：穿心莲内酯 白念珠菌 生物膜 

分 类 号：R379.4[医药卫生—病原生物学]