血管紧张素-（1-7）抑制肾小球系膜细胞增殖过程中c-fos蛋白表达  

Angiotensin-（1-7） inhibit the expression of c-fos in angiotensin Ⅱ-induced proliferative glomerular mesangial cells

作　　者：贺红焰[1] 沈宁[2] 刘建[2] 史小玲[3] 王明勇[3] 

机构地区：[1]泸州医学院病理教研室,四川泸州646000 [2]泸州医学院肾病内科,四川泸州646000 [3]泸州医学院分子与免疫研究室,四川泸州646000

出　　处：《中国医师杂志》2008年第7期868-870,共3页Journal of Chinese Physician

摘　　要：目的观察血管紧张素-（1-7）[Ang-（1-7）]对血管紧张素-Ⅱ（Ang-Ⅱ）诱导的肾小球系膜细胞（GMC）增殖过程中c-fos表达的影响。方法体外培养GMC，Ang-Ⅱ为刺激因子，不同浓度Ang-（1-7）为干预因子。用结晶紫计数法检测GMC数目的变化；Western blot检测GMC中c-fos蛋白表达的变化。结果Ang-（1-7）呈剂量依赖性地抑制Ang-Ⅱ诱导的GMC增殖和GMC内c-fos蛋白表达。结论Ang-（1-7）可能通过抑制c-fos的表达，抑制Ang-Ⅱ诱导的GMC增殖。Objective To investigate the effect of angiotensin-（1-7） on the expression of cellular c-fos in angiotensin Ⅱ-induced proliferative glomerular mesangial cells （GMC）. Methods GMC were treated with angiotensin Ⅱ and different dose of angiotensin-（ 1-7 ）.GMC number were evaluated by crystal violet staining and the expression of c-fos were detected by western blot..Results Angiotensin-（1-7）inhibit angiotensin II Ⅱ-induced GMC proliferation as well as the expression c-fos in a concentration dependent manner. Conclusion c-fos is involved in the inhibiting effects of angiotensin-（1-7） on angiotensin Ⅱ-induced GMC proliferation.

关 键 词：血管紧张素类 肾小球 细胞增殖 原癌基因蛋白质C-FOS 

分 类 号：R692.6[医药卫生—泌尿科学]