支气管哮喘IgE水平与β2-肾上腺素能受体基因多态性关系的研究  

Study on relationship between serum total IgE and β2-adrenoreceptor polymorphism in asthmatic patients

作　　者：叶贤伟[1] 冯端兴[1] 张湘燕[1] 余红[1] 刁晓源[1] 

机构地区：[1]贵州省人民医院呼吸内科贵州省呼吸疾病研究所,贵州贵阳550002

出　　处：《中国医师杂志》2008年第7期920-921,共2页Journal of Chinese Physician

基　　金：基金项目：贵州省科技攻关资助项目（黔科合2003NGY021）

摘　　要：目的探讨贵州汉族支气管哮喘人群血清总IgE水平与β-肾上腺素能受体（β2-AR）16、27位点基因多态性的相关性。方法44例支气管哮喘患者采用ELISA方法检测血清总IgE水平、等位基因特异性聚合酶链反应（AS-PCR）方法进行β2-AR基因多态性分析。结果β2-AR16位点的基因型Arg／Arg和Gly／Gly分布频率在IgE水平正常哮喘组、IgE水平升高哮喘组分别为17．4％、23．8％和43．5％、52．4％，呈递增趋势；Arg／Gly分布频率在两组中分别为39．1％、23．8％，呈递减趋势，但统计学分析差异无统计学意义；在27位点中，血清IgE水平升高的哮喘患者中，Gin／Gin纯舍予所占比例明显增高，达到76．2％；血清IgE水平不升高的哮喘患者中，Gln／Glu杂合予所占比例明显增高，达到73．9％；2组比较差异有统计学意义（P〈0．01）。结论血清总IgE水平与β2-AR27位点基因多态性相联，有助于贵州汉族支气管哮喘人群发病机制的进一步研究。Objective To study the association between serum total IgE level and β2-adrenoreceptor polymorphism （β2-AR） in asthmatic patients. Methods ELISA was used to determine serum total IgE and AS-PCR was used to determine β2- adrenoreceptor polymor- phism in 44 asthmatic patients. Results In β2- AR 16 locus genotype, the distribution frequencies of Arg/Arg, Gly/Gly showed increasing tendency, whereas Arg/Gly showed decreasing tendency, in normal serum total IgE group and increased serum total IgE group. But there was no significant difference between this two groups. In β2- AR 27 locus genotype, the distribution frequency of Gin/Gin genotype accounts for 76.2% and Gln/Glu genotype for 19.0% in increased serum total IgE group, while Gin/Gin genotype accounts for 9.0% and Gln/Glu geno- type for 73.9% in normal serum total IgE group. There was significant difference between two groups （ P 〈 0. 01 ）. Conclusions Serum total IgE was correlated with β2-AR 27 locus genetic polymorphism, which may contribute to understand the mechanism of asthma in the people of the Han nationality in GuiZhou.

关 键 词：哮喘 免疫球蛋白E 受体 肾上腺素能Β2 多态现象 遗传 

分 类 号：R562.25[医药卫生—呼吸系统]