农杆菌介导的几丁质酶基因转化花生的研究  

Studies on Transformation of Peanut with CH_5 B Gene Mediated by Agrobacterium tumefaciens

作　　者：张力凡[1] 王斌[2] 盖伟玲[1] 王晓杰[1] 乔利仙[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]中国科学院遗传与发育生物学研究所

出　　处：《青岛农业大学学报（自然科学版）》2008年第2期84-87,共4页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家公益性行业科研专项项目(nvhyzx07-014);国家自然基金项目(30270837)

摘　　要：以花生品种花育20成熟胚萌发5～7d的幼叶为外植体，利用农杆菌介导法进行几丁质酶基因（CH5B）的遗传转化，并对转化条件进行了研究。结果表明，外植体经预培养2d，于OD600=0．6～0．8的农杆菌（GV3101）菌液中浸泡15min，再共培养3d，最适宜遗传转化。对抗性植株进行PCR检测，扩增得到了目的条带，表明几丁质酶基因成功转入了花生。CH5B gene was transferred into peanut cultivar Huayu No. 20 mediated by Agrobacterium tumefaciens , and transformation conditions were studied in this paper. The result showed that pre - culture for 2 days, Agrobacterium suspension （OD600 =0.6-0.8） for 15 min and co-culture for 3 days was suitable to obtain resistant shoots. The target band of resistant plants was amplified by PCR analysis. These results confirmed that CH5B gene was transferred into peanut successfully.

关 键 词：花生 几丁质酶基因(CH5B) 农杆菌介导法 遗传转化 

分 类 号：Q789[生物学—分子生物学]