粗吻海龙蛋白质组分体外抗肿瘤活性的研究  

Study on the Anti-Tumor Effects of Proteins from Trachyrhamphus serratus in Vitro

作　　者：何芬[1] 马旭东[1] 王梦月[1] 秦孙星[1] 王威[1] 李晓波[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中国药学杂志》2008年第12期903-906,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目(30672620);上海-SK研究与发展基金项目(2003012-t)

摘　　要：目的探讨粗吻海龙水溶性蛋白质组分的抗肿瘤活性。方法应用四甲基偶氮唑盐法检测药物细胞毒效应,应用流式细胞术分析药物对A549细胞周期和凋亡率的变化,琼脂糖凝胶电泳、荧光显微镜观察药物对A549细胞凋亡和细胞周期相关性质改变。结果粗吻海龙蛋白对L1210、CCRF-CEM、A549、LOVO细胞的IC50分为1.86,1.56,0.88,1.74 g.L-1。粗吻海龙蛋白质组分对A549细胞作用表现出凋亡细胞特征性的改变,并能增强甲氨蝶呤、丝裂霉素对A549的细胞毒作用。结果粗吻海龙蛋白质组分对肿瘤细胞增生有明显的抑制作用,可引起肿瘤细胞凋亡。OBJECTIVE To study the anti-tumor activity of the water-soluble crude proteins from Trachyrhamphus serratus. METHODS MTT assay was used to evaluate the inhibitory effects of the crude proteins on proliferation of cancer cell lines. Cell cycle and apoptosis of A549 cells were analyzed by flow cytometry. Apoptosis induction was also proved by electrophoresis and morphologic assessment. RESULTS The IC50 values of the crude proteins for L1210、CCRF-CEM、A549、LOVO cells detected by MTT assay were 1.86,1.56,0. 88,1.74 g·L^-1, respectively. Moreover, the crude proteins induced apoptosis in A549 cells. At sub-cytotoxicity concentration, the crude protein showed synergistic effects with methotraexate （MTX） and mitomycin （ MMC ） in A549 cells. CONCLUSION The crude proteins from Trachyrhamphus serratus have anti-proliferation effect and induction of apoptosis in tumor cells in vitro.

关 键 词：粗吻海龙 蛋白质 抗肿瘤 凋亡 

分 类 号：R965[医药卫生—药理学]