RP-HPLC同时测定藏药波棱瓜子中7个活性木脂素成分的含量  

Simultaneous Determination of 7 Bioactive Lignans Compounds in Herpetospermum caudigerum by RP-HPLC

作　　者：丛龙波[1,2] 王琪[1,2] 黄雅洁[1] 袁海龙[1] 龚千锋[2] 杨明[2] 肖小河[1] 

机构地区：[1]解放军302医院,解放军中药研究所,北京100039 [2]中药现代制剂教育部重点实验室,江西中医学院,南昌330004

出　　处：《中国药学杂志》2008年第12期946-949,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立一种RP-HPLC同时测定藏药波棱瓜子中7个活性木脂素成分（ent-isolariciresinol,dehydrodiconiferyl alcohol,herpet-rione,herpetin,herpetetrone,herpetotriol,herpetal）的含量。方法采用Pinnacle DB C18（4.6 mm×250 mm,5μm,RESTEK）色谱柱,以乙腈-0.1%磷酸为流动相梯度洗脱,检测波长为240 nm,流速为1.0 mL·min^-1。结果Ent-isolariciresinol,dehydrodiconiferyl alcohol,herpetrione,herpetin,herpetetrone,herpetotriol和herpetal的线性范围分别为：1.29～24.80 mg·L^-1（r=0.978 9）,6.77～130.20 mg·L^-1（r=0.100 26）,10.41～200.20 mg·L^-1（r=0.980 2）,4.45～85.50（r=0.986 1）,2.91～56.10 mg·L^-1（r=0.982 8）,5.80～111.60mg·L^-1（r=0.100 78）和6.99～134.40 mg·L^-1（r=0.986 6）。平均回收率均在95.19%～102.64%内（RSDs〈1.52%）。结论本法准确可靠,灵敏度高,重现性好,结果表明,该分析方法适用于波棱瓜子药材的质量控制。OBJECTIVE To establish a method for the determination of 7 bioactive lignan compounds in seeds of Herpetospermum caudigerum, namely ent-isolariciresinol, dehydrodiconiferyl alcohol, herpetrione, herpetin, herpetetrone, herpetotriol, herpetal. METHODS The HPLC assay was performed on a RESTEK Pinnacle DB C18 column with gradient elution. The mobile phase consisted of acetonitrile and 0. 1% phosphoric acid at a flow rate of 1.0 mL·min^-1. The detection wavelength was 240 nm. RESULTS The linearity was obtained over the ranges of 1.29-24. 80 mg·L^-1 （ r = 0. 978 9） for ent-isolariciresinol, 6. 77-130. 20 mg·L^-1 （r =0. 100 26） for dehydrodiconiferyl alcohol, 10.41-200. 20 mg·L^-1 （r =0. 980 2） for herpetrione, 45-85.50 mg·L^-1 （r =0. 986 1 ） for herpetin, 2. 91-56. 10 mg·L^-1（ r = 0. 982 8 ） for herpetetrone, 5.80-111.60 mg·L^-1 （ r = 0. 100 78 ） for herpetotriol and 6. 99-134. 40 mg·L^-1 （r =0. 986 6） for herpetal, respectively. The recovery of the assay was in the range of 95. 19% -102. 64% with RSDs less than 1.52%. CONCLUSION The method is simple, sensitive and reproducible, and the results indicated that the developed assay could be considered as a suitable quality control method for Herpetospermum caudigerum.

关 键 词：波棱瓜子 RP-HPLC 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]