碱性成纤维细胞生长因子脂质体的制备和评价  

Preparation and Evaluation of bFGF-Encapsulated Liposomes

作　　者：黄亚东[1] 项琪[1] 张宏波 刘罕奇 苏志坚[1] 姚崇舜[1] 赵文[1] 

机构地区：[1]暨南大学医药生物技术研究开发中心,广州510632 [2]深圳致君制药有限公司,广东深圳518029 [3]南海朗肽制药有限公司,广东佛山528222

出　　处：《中国药学杂志》2008年第13期992-997,共6页Chinese Pharmaceutical Journal

基　　金：广东省科技攻关项目(2004B10410109);广东省科技攻关计划项目(2004A10902006)

摘　　要：目的考察碱性成纤维细胞生长因子脂质体最佳处方及工艺条件。方法以脂质体包封率、物理稳定性和生物活性为考察指标,采用单因素考察和全因素设计优化脂质体处方条件,正交设计优化脂质体制备工艺条件。结果最佳处方工艺条件下,碱性成纤维细胞生长因子(bFGF)脂质体包封率达(79.88±3.37)%,物理稳定性参数K_E为:(1.02±0.413)%;生物活性效价为:(6.147±0.769 1)×10~5U·mL^(-1)。结论制备工艺优化后的bFGF脂质体包封率高、稳定性好。OBJECTIVE To optimize the prescription and preparation of bFGF liposome. METHODS The encapsulation efficiency, stability KE; and biological activity of bFGF liposomes were evaluated by single factor test and full factor test. Orthogonal design were used to obtain the optimum prescription and preparation of bFGF liposome. RESULTS The encapsulation efficiency of optimal formulation was （79.88±3.37 ） %, stability KE was （ 1.02±0. 413 ） % and bioactivity was （6. 147±0. 769 1 ）×10^3 U·ml^-1. CONCLUSION bFGF lioposomes had high encapsulation efficiency,good physical chemical stability and high bioactivity.

关 键 词：碱性成纤维细胞生长因子 脂质体 正交设计 

分 类 号：R944[医药卫生—药剂学]