RP-HPLC同时测定菝葜中落新妇苷和黄杞苷的含量  

Simulataneous Determination of Astiibin and Engeletin in Smilax china by RP-HPLC

作　　者：周艳林[1] 钟小清[2] 吕高荣[2] 邹节明[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]桂林三金药业股份有限公司,广西桂林541004

出　　处：《中国药学杂志》2008年第14期1103-1105,共3页Chinese Pharmaceutical Journal

基　　金：广西优势中草药产业化资助项目(0444002-1)

摘　　要：目的建立同时测定菝葜药材中落新妇苷和黄杞苷含量的HPLC方法。方法采用Agilent Extend-C_(18)(4.6 mm×250 mm,5μm)柱,以甲醇-水-磷酸溶液(35:65:0.1)为流动相,流速1.0 mL·min^(-1),柱温35℃,检测波长为290nm。结果落新妇苷和黄杞苷分别在2.16×10^(-3)～1.08,3.176×10^(-3)～1.588μg内呈良好的线性关系;精密度试验RSD分别为0.75%,0.24%;重复性试验RSD分别为0.79%,0.89%;平均加样回收率分别为100.90%,98.71%。结论建立的方法可用于菝葜药材的质量控制。OBJECTIVE To establish a HPLC method for the determination of astilbin and engeletin in Smilax china. METHODS The samples were separated at 35 ℃ using a Agilent Extend-C18 column with methanol-water-phosphoric acid （35： 65： 0. 1 ） as mobile phase. Flow rate was at 1.0 mL ·min -1 and the detection wavelength was at 290 nm. RESULTS The calibration curves of astilbin and engeletin were linear over the ranges of 2. 16 × 10^-3- 1.08 and 3. 176 × 10^- 3 - 1. 588μg, RSDS of the pricision test were 0. 75% and 0. 24% , RSDS of the reproducibility were 0. 79% and 0. 89% , the average recoveries were 100. 90 % and 98.71%, respectively. CONCLUSION The method can be used as a quality control method for Smilax china.

关 键 词：菝葜 落新妇苷 黄杞苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]