柔嫩艾美耳球虫卵囊免疫后鸡盲肠扁桃体IL-2基因表达的动态变化  

Expressing profiling of IL-2 gene in cecal tonsil of chickens vaccinated with Eimeria tenella oocysts

作　　者：徐赓[1] 王彩霞[1] 王黎霞[1] 安健[1] 张鹏[1] 李卫东 

机构地区：[1]北京农学院动物科学技术系,北京昌平102206 [2]北京昌平城区动物卫生防疫站,北京昌平102200

出　　处：《中国兽医杂志》2008年第7期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金：北京市教委资助项目(KM20071002001);北京市属市管高校人才强教计划项目(PXM2007-014207-044539)

摘　　要：为检测和评价鸡白细胞介素-2(ChIL-2)在抗球虫免疫过程中的作用,以RT-PCR技术从鸡盲肠扁桃体中克隆获得了长度为391 bp的ChIL-2。将实验鸡随机分为对照组和试验组,试验组于12日龄和22日龄时用E.tenella孢子化卵囊进行免疫,分别于设定时间每组取5只鸡的盲肠扁桃体,以鸡β-Actin为参照建立半定量RT-PCR方法检测ChIL-2的表达动态变化。结果表明,试验组在第一次免疫后的第九天和第二次免疫后的第七天ChIL-2的表达量明显升高,提示ChIL-2参与了鸡体的抗球虫感染过程,为进一步探讨ChIL-2的生物学活性及作为鸡球虫病基因工程疫苗佐剂的研究奠定基础。In order to evaluate the effects of chicken interleukine-2 （IL-2）on the anticoccidial immunity, ChlL-2 were cloned from chicken cecal tonsil using RT-PCR with a pair of specific primers designed from the reported sequence in GenBank. The semi-quantitative RT-PCR using chicken 13-actin as internal control for detecting the expression of ChIL-2 were further developed and used for detecting the expression patterns of ChlL-2 in chickens vaccinated with sporulated oocysts of E. tenella at 12 and 22 day-old chickens. Our results indicate that the expression level of ChlL-2 in the experimental group significantly increased at day 9 post-primary and 7 post-secondary infections. It is suggested that ChlL-2 can play important roles in the immune responses against E. tenella infection in chickens.

关 键 词：鸡 盲肠扁桃体 IL-2 半定量RT-PCR 

分 类 号：Q786[生物学—分子生物学]