硝酸甘油间断性给药对大鼠糖皮质激素性骨质疏松模型骨密度及血清NOS水平影响的初步研究  

Effect of intermittent administration of Nitroglycerin on BMD and serum NOS levels in glucocorticoid-induced osteoporosis

作　　者：颜廷振[1] 黄朝梁[1] 邓忠良[1] 陈世荣[1] 柯珍勇[1] 蔺超[1] 

机构地区：[1]重庆市渝中区临江路重庆医科大学附属第二医院骨科,重庆市400016

出　　处：《中国骨质疏松杂志》2008年第7期482-485,470,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察大鼠糖皮质激素性骨质疏松模型血清一氧化氮合酶(NOS)变化及硝酸甘油(Nitroglycerin,NG)对糖皮质激素性骨质疏松的防治作用。方法健康3月龄SD雄性大鼠30只,体重(251±12.5)g,随机分为3组,每组10只。模型组(A)给予生理盐水和地塞米松,实验组(B)给予地塞米松和硝酸甘油,对照组(C)给予生理盐水。试验8周后测量各组大鼠第3腰椎及右股骨中段骨密度,并行切片HE染色观察骨组织显微结构,测量大鼠体重、血清一氧化氮合酶(NOS)、碱性磷酸酶含量。结果8周后3组体重差异明显(P<0.01),模型组体重低于实验组,实验组低于对照组。实验组第3腰椎骨密度值明显高于模型组(P<0.01)。对照组骨密度值与实验组比较差异无显著性意义(P>0.05)。各组股骨骨密度值无明显差异(P>0.05)。实验组第3腰椎骨小梁形态结构与对照组相似,模型组骨小梁稀疏,断裂。模型组ALP较对照组及实验组明显升高(P<0.01),NOS各组差异不明显(P>0.05)。结论血清NOS水平不宜作为激素性骨质疏松症的评价指标,硝酸甘油可有效预防糖皮质激素性骨质疏松的发生。Objective To observe the effect of NG on serum NOS levels in rat model with glucocorticoid-induced osteoporosis and its effectiveness on preventing glucocorticoid-induced osteoporosis. Methods Thirty male SD rats, weighing（251 ~ 12.5 g） each, were randomly divided into 3 groups （10 rats for each group）. The experiment group A rats were given natrium chloride and dexamcthasone. The group B were given dexamcthasone and NG. Control group C rats were given only natrium chloride. Eight weeks later, the bone mineral density of the third lumbar vertebral bodies and the femur of all the rats were measured, and the structure of bone trabeculae, the body weight, NOS, ALP in serums were measured too. Results The values of bone mineral density in Group C were significantly higher than that of Group B, （ P 〈 0.01 ）. No significant difference was observed in bone mineral density between Group A and Group C （P 〉 0.05 ）. The bone mineral density of femur between the groups presented no significant difference. The structure of bone trabeculae of Group C was similar to that of Group B, and Group A presented the feature of osteoporosis. ALP levels of Group A were significantly increased compared with the corresponding value of Group B and Group C（P 〈 0.01） The serum NOS between the three groups was of no significant difference（ P 〉 0.05） Conclusion The change of serum NOS is not suitable for evaluating the bone loss in rat model with glucocorticoid-induced osteoporosis. Intermittent administration of NG can prevent the osteoporosis induced by gnlcocorticoid effectively.

关 键 词：骨质疏松症 糖皮质激素 硝酸甘油 一氧化氮合酶 

分 类 号：R681[医药卫生—骨科学]