早孕小鼠子宫内膜钙网蛋白的表达规律  

Expression of Calreticulin in the Endometrium of Early Pregnant Mouse

作　　者：董艳玲[1] 何俊琳[1] 刘学庆[1] 丁裕斌[1] 黄德培[1] 王应雄[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生物学研究室,遗传优生教研室,重庆400016

出　　处：《细胞生物学杂志》2008年第3期372-376,共5页Chinese Journal of Cell Biology

基　　金：重庆市自然科学基金重点资助项目(渝科发计字[2004]47号)~~

摘　　要：采用RT-PCR、间接免疫荧光组织化学、Western印迹及原位杂交技术分别检测未孕(d0)和妊娠d1、d2、d3、d4、d5、d6、d7天小鼠子宫内膜中钙网蛋白(calreticulin,CRT)的表达规律,探讨CRT在胚胎着床中的作用。结果显示CRT mRNA在妊娠小鼠子宫内膜中的表达明显高于未孕小鼠(P<0.05),且随着妊娠天数的增加呈逐渐增强的趋势。间接免役荧光组织化学结果显示CRT表达于子宫内膜基质细胞、腺上皮以及腔上皮,并在妊娠第4、5天基质细胞的胞浆中呈现高峰。实验结果提示,CRT在妊娠早期子宫内膜的持续表达,可能通过调节整合素介导的细胞信号通路而调节胚胎滋养层细胞的黏附、侵袭,参与胚胎着床。To probe the role of calreticulin （CRT） in blastocyst implantation, we have detected expression of CRT mRNA and the protein in endomewia from mice of non-pregnancy and pregnancy on days 1, 2, 3, 4, 5, 6, 7, respectively by RT-PCR, indirect immunofluorescence histochemistry, Western blot and in situ hybridization techniques. The results showed that the expression of CRT mRNA and the protein is higher in pregnant mice than that of non-pregnant mice, and reach maximal level pregnant on day 4, 5 and was located in the uterine stromal cells and epithelium. The result suggested that the expression characteristic of CRT might involved in adhesion/invasion of the blastocyst to endometrium and be useful for embryo implantation and maintenance of pregnancy in mouse.

关 键 词：钙网蛋白 胚胎着床 小鼠 

分 类 号：R714[医药卫生—妇产科学]