白纹佛蝗线粒体全基因组序列(英文)  

Complete Sequencing and Analysis of mtDNA in Phlaeoba albonema Zheng

作　　者：师红雯[1] 丁方美[1] 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《中国生物化学与分子生物学报》2008年第7期604-611,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No30470238)资助~~

摘　　要：通过长PCR扩增线粒体全基因组进行保守引物步移法结合克隆测序技术,对白纹佛蝗mtDNA全序列进行了测定和分析.结果表明：白纹佛蝗线粒体基因组全长15 657 bp,包含13个蛋白编码基因、22个tRNA基因和2个rRNA基因以及1个非编码的控制区域,它们的长度分别是11 202 bp,1 486 bp,2 156 bp和728 bp.37个基因的位置与飞蝗的一致,有9对基因间存在41 bp重叠,重叠碱基数在1 ～8 bp之间;基因间隔序列共计21处126 bp,间隔长度从1 ～20 bp不等,最大的基因间隔是20 bp,是在tRNALys和ATP8基因之间.还对lrRNA和srRNA二级结构进行了预测,同时也对tRNA反密码子臂的碱基对类型以及不同链上蛋白编码基因的A/T,C/G组成偏向性进行了详细的讨论.The complete mitochondrial genome sequence of Phlaeoba albonema Zheng was determined by using L-PCR and conserved primers walking sequencing. The obtained genome sequence is 15 657 bp in size, containing 13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNA genes. All the 37 genes are conserved in the same orientations as observed in Locusta migratoria. 11 202 bp of the mtDNA are coding for proteins, 1 486 bp for tRNAs, 1 312 bp for rRNA large subunit （lrRNA）, and 844 bp for rRNA small subunit （srRNA）. The A ＋ T-rich region is 728 bp in size. The genes overlapping sequences are gl bp in total and are spreading over 9 locations （1-8 bp at each site）. A total of 126 bp intergenic spacer sequences are scattered in 21 regions at the size of 1 to 20 bp, where the largest 20 bp region is located between the tRNA^Lys and ATP8 genes. The predicted secondary structures of both srRNA and lrRNA were compared with that of Ruspolia dubia, and the patterns of base pairs in tRNA anticodon stem and A/T, C/G bias of protein-coding genes in different strands were discussed.

关 键 词：线粒体基因组 rRNA二级结构 TRNA基因 链偏向性 

分 类 号：Q96[生物学—昆虫学] Q78