慢性多重应激大鼠ECG变化和下丘脑-垂体-肾上腺轴应激反应增高  

Effect of chronic multi-stress on ECG and HPA axis activity in rats

作　　者：李福平[1] 高凌云[2] 肖颖彬[1] 

机构地区：[1]第三军医大学新桥医院心血管外科,重庆400037 [2]第三军医大学新桥医院心血管内科,重庆400037

出　　处：《心脏杂志》2008年第4期414-416,共3页Chinese Heart Journal

基　　金：全军医学科学技术研究"十一五"计划课题资助(06H023)

摘　　要：目的研究慢性多重应激大鼠脱离应激源后心电图(Electrocardiogram,ECG)变化和下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenocortical,HPA)轴应激反应。方法健康雄性SD大鼠20只随机分为应激组和对照组。选用刺激脉冲随机变动的噪声、足底电击、强迫游泳、束缚和夜间光照的复合刺激为应激组大鼠应激源,应激刺激持续30d,建立大鼠慢性多重应激模型。于应激刺激前和完全脱离应激源后3d记录心电图,分析血清促肾上腺皮质激素(ACTH)和皮质酮(corticosterone,Cor)的变化。结果刺激4周后,脱离应激源3d,在1h心电图记录期中,大鼠心律失常类型和次数明显多于应激前和对照组:持续窦性心动过速或窦性不齐、房性期前收缩(应激前1.4±0.7vs应激后40.2±5,P<0.01)、室性期前收缩(应激前3.1±0.6vs应激后55.7±4,P<0.01)、阵发性室速(应激前0vs应激后23.8±6,P<0.01)。心率明显增快[(371.3±12)次/min→(508.7±10)次/min,P<0.01]、RR间期明显缩短[(171.6±5)ms→(111.5±0.5)ms,P<0.01]、ST段抬高或下移、T波高耸直立或低平,血清ACTH[(53.1±4)ng/L→(234.7±16)ng/L,P<0.01]和Cor[(16±4)μg/L→(279±50)μg/L,P<0.01]水平明显增高。结论慢性多重应激大鼠在脱离应激源后仍存在心律失常,并且HPA轴应激反应增高。AIM To study the effect of chronic multi-stress on ECG and HPA axis activity in rats after exposure to multiple stressors. METHODS In this study a chronic multi-stress animal model was established in rats and the animals were subjected to 4 weeks of chronic multi-stress. Electrocardiographic recordings were performed before （baseline, B） and after （poststress, PS） chronic multi-stress. HPA axis activity was examined by collecting blood samples, which were analyzed for plasma ACTH and corticosterone （Cor） concentrations. The effect of chronic multi-stress on electrocardiogram （ECG） and serum ACTH and Cor was analyzed. RESULTS After 4 weeks of chronic multi-stress, a much higher incidence of arrhythmias including sinus tachycardia, sinus arrhythmia, atrial premature beats （ B ： 1.4 ± 0. 7 vs PS： 40. 2 ±5, P 〈0. 01 ）, ventricular premature beats（ B：3. 1 ±0. 6 vs PS： 55.7 ±4, P 〈0. 01 ） and ventricular tachycardia （ B ： 0 vs PS ： 23.8 ± 6, P 〈 0. 01 ） was produced. The chronic multi-stress also induced a much stronger increase of heart rate [ （ 371.3 ± 12 ）/min→（ 508. 7 ± 10 ）/min, P 〈 0. 01 ] and a reduction of average R-R interval [ （ 171.6 ± 5 ） ms→（ 111.5 ± 0. 5 ） ms, P 〈 0. 01 ]. The ST of ECG was lightly displaced, T wave of ECG was upright lofty and serum ACTH [ （53.1 ± 4） ng/L→（234.7 ±16） ng/L, P〈0.01], Cor [（16 ±4） μg/L→（279 ±50） μg/L, P〈0.01] level was raised compared with those in baseline and the control groups. CONCLSUSION Chronic multi-stress causes ar rhythmias and increases HPA axis activity in rats following their exposure to multiple stressors.

关 键 词：慢性多重应激 心电图 下丘脑-垂体-肾上腺轴 

分 类 号：R540.41[医药卫生—心血管疾病]