黄芪注射液对兔肠系膜上动脉闭塞性休克脂质过氧化损伤的防治及其机制  

Effect of astragalus injection on lipid peroxidiation associated with superior mesenteric artery occlusion shock in rabbits

作　　者：王艳蕾[1] 景友玲[1] 李宏杰[1] 张一兵[1] 段国贤[1] 王小君[1] 张伟[1] 赵春秀[1] 

机构地区：[1]华北煤炭医学院生理教研室,河北省唐山市063000

出　　处：《世界华人消化杂志》2008年第18期2040-2044,共5页World Chinese Journal of Digestology

基　　金：河北省煤炭厅基金资助项目;No.96141600451~~

摘　　要：目的:探讨黄芪注射液对肠系膜上动脉闭塞性(superior mesenteric artery occlusion,SMAO)休克脂质过氧化损伤的防治作用及其机制.方法:经腹分离兔肠系膜上动脉并夹闭2h后松夹,复制SMAO休克动物模型,并于松夹前、后各15min分别将黄芪注射液1mL/kg以2倍的NS配制自耳缘iv,观察动物血压、血浆及红细胞膜丙二醛(MDA)、红细胞膜微黏度、红细胞超氧化物歧化酶(SOD)、血浆黄嘌呤氧化酶(XOD)、乳酸脱氢酶(LDH)及酸性磷酸酶(ACP)含量的变化;并进行小肠组织病理学检查.结果:与对照组比较,SMAO组血压和红细胞SOD降低(均P<0.01),红细胞膜微粘度、红细胞膜MDA以及血浆MDA,XOD,LDH和ACP的水平明显升高(均P<0.01),光镜下小肠病理损害明显.黄芪注射液治疗后上述各指标均较SMAO组明显改善(80.1±3.6 vs 39.4±5.2,4.63±0.57 vs 3.44±0.61,3.35±0.34 vs 4.09±0.38,0.23±0.02 vs 0.41±0.02,3.61±0.41 vs 4.32±0.92,71.4±13.1 vs 92.5±13.9,50.2±18.2 vs 105.5±37.0,37.0±11.8 vs 71.7±22.0,均P<0.01).结论:SMAO休克伴有氧自由基代谢紊乱,体内脂质过氧化过程加强.黄芪注射液通过抗脂质过氧化稳定细胞膜,改善红细胞膜微黏度,减轻组织损伤,延缓SMAO休克的发展.AIM： To study preventive and therapeutic effect of astragalus injection on lipid peroxidiation associated with superior mesenteric artery occlusion （SMAO） shock as well as its mechanism. METHODS： SMAO shock model in rabbits was induced by occlusion of the superior mesenteric artery for 2 h. Astragalus injection （1 mL/kg） dissolved with normal saline was administrated iv. through marginal vein of ear 15 minutes before shock and 15 minutes after shock. Blood pressure, plasma and erythrocyte membrane malondialdehyde （MDA）, plasma oxidase （XOD）, lactate dehydrogenase （LDH） and acidphosphatase （ACP） in plasma, erythrocyte superoxide dismutase （SOD） and erythrocyte membrane microviscosity were determined. Intestinal histology was examined under light microscopy. RESULTS： Compared with those in sham operation group, the pressure and erythrocyte SOD were lower in SMAO group （both P 〈 0.01）;the changes of erythrocyte membrane viscosity, MDA, XOD, LDH and ACP were markedly higher in SMAO group than sham group （both P 〈 0.01）; and severe damages of intestinal tissues were observed under light microscopy. The indexes mentioned above in astragalus group were obviously ameliorated in comparison with those in SMAO group （80.1 ± 3.6 vs 39.4 ± 5.2, 4.63 ± 0.57 vs 3.44 ± 0.61, 3.35 ± 0.34 vs 4.09 ± 0.38, 0.23 ± 0.02 vs 0.41 ± 0.02, 3.61 ± 0.41 vs 4.32 ± 0.92, 71.4 ± 13.1 vs 92.5 ± 13.9, 50.2 ± 18.2 vs 105.5 ± 37.0, 37.0 ± 11.8 vs 71.7 ± 22.0, all P 〈 0.01）. CONCLUSION： Metabolic confusion of oxygen free radicals and increased lipid peroxidation occur in the course of SMAO shock. Astragalus injection, through anti-lipid peroxidation and free radical removal, stabilizes cell membrane, improves erythrocyte membrane microviscosity, alleviates tissue injury, delays SMAO shock development and has preventive and therapeutic effects on SMAO shock.

关 键 词：黄芪注射液 肠系膜上动脉闭塞性休克 脂质过氧化 红细胞膜微黏度 

分 类 号：R285.5[医药卫生—中药学]