慢性HBV感染产妇胎儿脐带血单核细胞PD-L1的表达  

PD-L1 expression in umbilical cord blood monocytes of neonates born from chronic HBV-infected mothers

作　　者：于贤杰[1] 刘敏[2] 杨慧霞[3] 朱曼华[1] 陈姬[1] 吴学杰[1] 王贵强[1] 

机构地区：[1]北京大学第一医院感染疾病科,北京市100034 [2]北京地坛医院妇产科,北京市100011 [3]北京大学第一医院产科,北京市100034

出　　处：《世界华人消化杂志》2008年第18期2061-2064,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30771905~~

摘　　要：目的:检测慢性HBV感染产妇胎儿脐带血单核细胞PD-L1表达情况,探讨脐血清HBeAg与PD-L1表达的关系.方法:采用流式细胞仪技术检测31例HBsAg阳性产妇和10例健康产妇胎儿脐带血单核细胞PD-L1的表达,微粒子免疫荧光法(MEIA)检测脐血清HBeAg.结果:HBeAg阴性产妇组胎儿脐带血单核细胞PD-L1表达与健康产妇组无显著差异,而HBeAg阳性组中,3例脐血清HBeAg高水平患者,脐带血单核细胞PD-L1表达显著高于HBeAg低水平组、HBeAg阴性组及健康产妇组(22.0±1.6 vs 2.55±3.1,1.3±1.2,1.5±0.8,均P=0.00);HBeAg量处于低水平者,其单核细胞PD-L1表达与HBeAg阴性者及健康产妇组比较无显著差异.结论:慢性HBV感染产妇胎儿脐血高水平HBeAg可能和单核细胞PD-L1高表达有关,可能和新生儿HBV感染慢性化机制有关.AIM： To detect PD-L1 expression in umbilical cord blood monocytes of neonates born from chronic HBV-infected mothers and to determine relationship between serum HBeAg and PD-L1 expression. METHODS： Cord blood mononuclear cells （CBMC） were collected from umbilical cord of cesarean-delivered neonates born from 31 HBsAg- positive mothers and 10 healthy mothers. CBMC were stained with CD14-FITC and PD-L1-APC to detect the expression rate of PD-L1 using flow cytometry. Cord blood serum HBeAg was detected using microparticle enzyme immunoassay （MEIA）. RESULTS： There was no significant differencewith PD-L1 expression in cord blood monocytes between neonates born from HBeAg-negative mothers and those born from healthy mothers. In three cases of chronic HBV carrier mothers with high HBeAg level, PD-L1 expression in cord blood monocytes was significantly higher than that of other groups （22.0 ± 1.6 vs 2.55 ± 3.1, 1.3 ± 1.2, 1.5 ± 0.8, all P = 0.00）. Compared with the PD-L1 expression of low HBeAg levels, there were no significant difference between HBeAg-negative mother group and healthy mother group. CONCLUSION： High HBeAg levels in umbilical cord blood may be related to up-regulation of PD-L1 in cord blood monocytes of neonates born from HBeAg-positive mothers. It might be related to neonatal persistent HBV infection.

关 键 词：程序性细胞死亡分子1 慢性HBV感染 脐带血单核细胞 HBEAG 感染慢性化 

分 类 号：R714.2[医药卫生—妇产科学] R512.62[医药卫生—临床医学]