白藜芦醇下调小鼠非乙醇性脂肪性肝炎环氧合酶2的表达  

Resveratrol down-regulates hepatic cyclooxygenase-2 expression in mice with nonalcoholic steatohepatitis

作　　者：肖虎[1] 张皎月[1] 郑涓[1] 

机构地区：[1]华中科技大学协和医院内分泌科,湖北省武汉市430022

出　　处：《世界华人消化杂志》2008年第19期2092-2096,共5页World Chinese Journal of Digestology

基　　金：湖北省科技攻关计划;No.2007AA302B05~~

摘　　要：目的:研究白藜芦醇对饮食诱导的非乙醇性脂肪性肝炎(NASH)小鼠肝脏COX-2表达的影响.方法:♂小鼠30只随机分为正常对照组(NC组,n=10)、高脂喂养组(HF组,n=10)和高脂喂养白藜芦醇治疗组(HR组,n=10).NC组给予标准基础饲料,HF组和HR组给予高脂饲料喂养.HR组小鼠高脂喂养8wk后,每日给予400mg/kg白藜芦醇灌胃治疗.HR组和HF组均继续高脂饲料喂养16wk.实验结束后处死小鼠并取肝脏组织,分别用RT-PCR法及免疫印迹法检测肝脏COX-2mRNA和蛋白的表达.结果:HF组小鼠出现明显的脂肪性肝炎,HR组小鼠的脂肪性肝炎明显减轻.NC组小鼠肝组织无COX-2mRNA和蛋白的表达,HF组小鼠肝组织有高水平的COX-2mRNA和蛋白的表达,分别为1.48%±0.23%和27.9%±4.6%,HR组COX-2mRNA和蛋白的表达水平较HF组明显降低,分别为0.76%±0.18%和11.2%±3.5%,两组间差异有统计学意义(P<0.01),但未降至正常.结论:COX-2在NASH中发挥着重要作用,白藜芦醇至少部分是通过下调肝脏COX-2的表达达到改善NASH的作用.AIM： To investigate the effect of resveratrol on gene expression of cyclooxygenase （COX）-2 in mice with diet-induced nonalcoholic steatoheptatitis （NASH）. METHODS： Thirty male C57BL/6J mice were assigned randomly to three groups： normal control group, high fat group （HF group） and high fat resveratrol treatment group （HR group）. The mice in normal control group （n = 10） were fed with standard diet. The mice in HF group （n = 10） and in HR group （n = 10） were fed with high fat diet. After 8-week high fat diet, the mice in HR group were fed with resveratrol 400 mg/（kg·day） for 16 wk. Hepatic COX-2 expression was determined using RT- PCR and Western blot. RESULTS： Steatoheptatitis was discernable in HF group and was markedly alleviated in HR group. COX-2 were not expressed in normal mouse liver. The mRNA expression and protein expression of COX-2 were significantly lower in HR group than those in HF group（0.76% ± 0.18% vs 1.48% ±0.23%, 11.2% ± 3.5% vs 27.9% ± 4.6%, both P 〈 0.01）, but not returned to normal level. CONCLUSION： COX-2 may play an important role in NASH pathogenesis. Resveratrol improves NASH at least partly by downregulating expression of COX-2 in the liver .

关 键 词：脂肪肝 非乙醇性脂肪性肝病 环氧合酶2 白藜芦醇 

分 类 号：R285.5[医药卫生—中药学]