小檗碱对3T3-L1胰岛素抵抗细胞模型PI-3K p85蛋白表达的影响  

Effects of berberine on PI-3K p85 protein expression in insulin-resistant cell model in 3T3-L1 adipocytes

作　　者：易屏[1] 陆付耳[2] 陈广[2] 徐丽君[2] 董慧[2] 王开富[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中医科,湖北省武汉市430030 [2]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北省武汉市430030

出　　处：《世界华人消化杂志》2008年第19期2102-2106,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30371816~~

摘　　要：目的:研究小檗碱对3T3-L1胰岛素抵抗细胞模型PI-3K p85蛋白表达的影响,探讨小檗碱改善胰岛素抵抗的分子机制.方法:分别以0.5mmol/L软脂酸与25mmol/L葡萄糖加0.6nmmol/L胰岛素诱导3T3-L1脂肪细胞产生胰岛素抵抗,予以小檗碱进行干预,同时以阿司匹林作为阳性对照,以2-脱氧-[3H]-D-葡萄糖摄入法观察葡萄糖的转运率,用Western blot检测PI-3K p85蛋白的表达.结果:0.5mmol/L软脂酸作用24h或25mmol/L葡萄糖加0.6nmmol/L胰岛素作用18h分别使3T3-L1脂肪细胞胰岛素刺激的葡萄糖转运抑制67%和60%,Western blot显示PI-3K p85蛋白表达减少,与正常对照组比较有统计学意义(P<0.01);同时加入小檗碱则可逆转上述效应使PI-3K p85蛋白表达增加,与模型组比较有明显差异(P<0.01),并且PI-3K p85蛋白的表达与小檗碱的剂量和作用时间呈依赖关系.结论:小檗碱可明显改善游离脂肪酸和高糖诱导的胰岛素抵抗,其分子机制可能与小檗碱提高PI-3K p85蛋白的表达有关.AIM： To investigate the effects of berberine on PI-3K p85 protein expression in insul＇m-resistant cell model in 3T3-L1 adipocytes and its possible molecular mechanism. METHODS： 3T3-L1 adipocytes were treated with 0.5 mmol/L palmic acid or with 25 mmol/ L glucose in combination with 0.6 nmol/L insulin to induce insulin resistance. Berberine was used for treatment and aspirin was used for positive control. 2-deoxy-[^3H]-D-glucose method was used for determination of glucose uptake. Western blot was performed to estimate protein expression of PI-3K p85. RESULTS： After the intervention of palmic acid for 24 h or 25 mmol/L glucose with 0.6 nmol/L insulin for 18 h, the insulinstimulated glucose transport in 3T3-L1 adipocytes was inhibited by 67% and 60% respectively. Meanwhile, the expression of PI-3K p85 protein was significantly reduced compared with normal control group （P 〈 0.01）. However, berberine reversed above phenomenon and significantly enhanced PI-3K p85 protein expression （P 〈 0,01）, The expression rate of PI-3K p85 protein was time- and dosedependent on berberine, CONCLUSION： Insulin resistance induced by FFAs or high glucose in 3T3-L1 adipocytes can be improved by berberine and its molecular mechanism might be associated with the expression of PI-3K p85.

关 键 词：小檗碱 胰岛素抵抗 PI-3K P85 游离脂肪酸 高糖 

分 类 号：R285.5[医药卫生—中药学]