肠炎宁糖浆对内脏高敏感大鼠脑、脊髓CRF表达的影响  

Effect of Changyanning Tangjiang on the expression of corticotropin releasing factor in spinal cord and brain of rats with visceral hypersensitivity

作　　者：晁冠群[1] 吕宾[1] 孟立娜[1] 张烁[1] 张璐[1] 郭赟[1] 

机构地区：[1]浙江中医药大学附属第一医院消化内科,浙江省杭州市310006

出　　处：《世界华人消化杂志》2008年第19期2112-2118,共7页World Chinese Journal of Digestology

摘　　要：目的:研究内脏高敏感大鼠的脊髓、脑部CRF的分布和表达,探讨CRF在肠易激综合征(IBS)内脏高敏感信号传导通路中的机制,以及肠炎宁糖浆对IBS起效的可能作用机制.方法:清洁级成年♀SD大鼠40只,随机分3组,空白组(n=8),模型一组(腹腔注射鸡卵清蛋白致敏,n=16),模型二组(条件刺激和非条件刺激轮替致敏,n=16),评估肠道敏感性后,模型一组和模型二组均随机分成2组,即对照组和肠炎宁组,每组8只.空白组和对照组给予生理盐水,肠炎宁组给予肠炎宁,ig4wk,取脑、脊髓进行免疫组化,观察CRF的分布和表达情况.结果:肠炎宁组内脏敏感性较模型组明显降低(P<0.01).免疫组化显示大鼠下丘脑、第三脑室下侧、脊髓腰膨大部可见CRF的明显表达,CRF阳性指数模型一对照组和模型二对照组均高于空白对照组,差异有统计学意义(下丘脑:0.037±0.009,0.037±0.024vs0.005±0.001;第三脑室下侧:0.038±0.009,0.040±0.022vs0.005±0.001;脊髓:0.028+0.008,0.024±0.004vs0.002±0.001;均P<0.01).模型一肠炎宁组(0.012±0.005,0.012±0.005,0.010±0.003)较模型一对照组高,模型二肠炎宁组(0.009±0.005,0.011±0.006,0.012±0.005)较模型二对照组高,模型一肠炎宁组和模型二肠炎宁组较空白对照组高,差异均有统计学意义(P<0.05).结论:CRF在内脏刺激信号的传入过程中起重要作用,肠炎宁可以降低其表达程度,这可能是降低大鼠内脏高敏感的机制之一.AIM： To investigate the distribution and expression of corticotropin releasing factor in spinal cord and brain of rats with visceral hypersensitivity, and to explore the mechnism of Changyanning Tangjiang treating irritable bowel syndrome. METHODS： Forty female SD rats were divided randomly into 3 groups： blank control group （n = 8）, model I group （n = 16, sensitized with intraperitoneal injection of chicken ovalbumin） and model 2 group （n = 16, sensitized in turn with conditional stimulation and non-conditional stimulation）. After the intestinal hypersensitivity was evaluated, the rats in the two model groups were divided into 2 subgroups： model control group （n = 8） and Changyanning group （n = 8）.The rats in the blank control group and model group were treated with normal saline, and those in Changyanning group were intragastrically treated with Changyanning Tangjiang for 4 wk. Then spinal cord and brain were collected for im- munohistochemical staining, and the distribution and expression of CRF were observed. RESULTS： The visceral sensitivity of rats in the Changyanning groups was lower than that in the model groups （P 〈 0.01）. Immunohistochemistry showed that CRF was obviously expressed in the lumbar intumescentia of spinal cord, hypothalamus and diaphragmatic surface of the third ventricle of cerebrum. The positive indexes in the model control groups were higher than those in the blank control group （hypothalamus： 0.037 ± 0.009, 0.037 ± 0.024 vs 0.005± 0.001; diaphragmatic surface： 0.038± 0.009, 0.040 ± 0.022 vs 0.005 ± 0.001; spinal cord： 0.028 ±0.008, 0.024 ± 0.004 vs 0.002 ±0.001; all P 〈 0.01）. The positive indexes in the Changyanning groups （hypothalamus： 0.012± 0.005, 0.009 ± 0.005; diaphragmatic surface： 0.012± 0.005, 0.011 ±0.006; spinal cord： 0.010 ± 0.003, 0.012 ± 0.005） were lower than that in the model groups. The positive index of CRF was also higher in the Changyanning groups than that in the blank control group （P

关 键 词：促肾上腺皮质激素释放因子 内脏高敏感 下丘脑 第三脑室 脊髓 

分 类 号：R285.5[医药卫生—中药学]