瑟伯氏棉和异常棉的陆地棉导入系的EST-SSR和gSSR分析(英文)  

Characteristics of Gossypium thurberi and G.anomalum Introgression Lines of G.hirsutum Revealed by EST-SSR and gSSR

作　　者：林忠旭[1] 王锦峰[1] 张献龙[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070

出　　处：《棉花学报》2008年第4期243-248,共6页Cotton Science

基　　金：Professor NIU Yong-zhang(Agricultural Science Institute of Shanxi Province)provides the upland accessions,DH961-DH968;973 Project(No.2004CB117301)~~

摘　　要：用EST-SSR和gSSR分析了瑟伯氏棉和异常棉的8个导入系。结果表明,SSR的扩增带可以分为两大类:第一大类是导入系和受体晋棉6号之间没有差异;第二大类是导入系和受体晋棉6号之间存在差异。第一大类可分为5个小亚类;第二大类中,对于EST-SSR来说,可分为4个小亚类:一是在晋棉6号中出现的条带在部分导入系中消失;二是条带大小与晋棉6号相比有变化;三是部分导入系中出现了瑟伯氏棉和异常棉的条带,但与晋棉6号相同的条带消失了;四是部分导入系中出现了供体和受体都没有的特异条带,而在另一些导入系中条带大小发生变化。对于gSSR来说,第二大类中又多了两个小亚类:即部分导入系中除了供体和晋棉6号都没有的条带;或供体和晋棉6号的条带同时出现在导入系中。这些供体和受体异常条带的增加和消失可能是SSR区域和SSR侧翼序列变化的结果。与gSSR相比,EST-SSR在导入系和晋棉6号之间的变化更大,这可能是导致导入系比晋棉6号纤维品质更优的原因。Eight introgression lines （ILs） of G. thurberi and G. anomalum were analyzed using EST- SSR and gSSR. The results showed that the bands detected by SSR markers could be sorted into two classes： （Ⅰ） no differences between the eight ILs and Jinmian 6, the upland cotton receptor, and （Ⅱ） differences among the eight ILs and Jinmian 6. In class Ⅰ, five sub-classes can be identified. In class Ⅱ, four sub-classes could be identified for EST-SSR： （1） bands detected in Jinmian 6 but absent in some ILs; （2） band size changed compared to Jinmian 6; （3） bands that introgressed from G. thurberi and G. anomalum while bands same as Jinmian 6 disappeared in some ILs; （4） the novel bands that differ from either the donor or the receptor appeared in some ILs, while bands size changed compared to Jinmian 6 in some other ILs. For gSSR, two additional sub-classes could be identified; （5） additional bands appeared in some ILs; （6） both donors＇ and Jinmian 6＇s bands appeared in ILs. The appearance of novel bands or disappearance of parental bands may result from the changes of SSR region and flanking sequence. Compared to the gSSRs, EST-SSRs were more variable between the ILs and Jinmian 6, which may be a result of the introgressed good fiber quality of the ILs.

关 键 词：导入系 瑟伯氏棉 异常棉 EST—SSR gSSR 

分 类 号：S562[农业科学—作物学]