支扩宁合剂对人支气管上皮细胞肿瘤坏死因子-α表达影响的体外研究  

Effect of Zhikuoning Mixture on Expression of TNF-α in Human Bronchial Epithelial Cells in vitro Study

作　　者：吴其标[1] 曹世宏[1] 卢金福[2] 达庆国[3] 孙子凯[3] 

机构地区：[1]澳门科技大学,中国澳门 [2]南京中医药大学,江苏南京210029 [3]南京中医药大学附属医院,江苏南京210029

出　　处：《时珍国医国药》2008年第4期788-789,共2页Lishizhen Medicine and Materia Medica Research

基　　金：澳门科学技术发展基金资助项目(No.003/2005/A);江苏省高校自然科学研究计划;南京中医药大学青年科技创新基金资助项目(No.05XZR03)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)在支气管扩张症发病机制中的作用及支扩宁合剂的疗效机理。方法应用支气管扩张症患者痰液刺激分组体外培养的人支气管上皮细胞,检测、分析各组细胞TNF-α表达水平及中药支扩宁合剂的干预效果。结果在支气管扩张症患者的痰液刺激下,支气管上皮细胞TNF-α表达活化;中药支扩宁合剂可抑制支气管上皮细胞TNF-α的表达。结论支气管上皮细胞TNF-α表达在支气管扩张症发病中可能起到重要的作用;中药支扩宁合剂可抑制支气管上皮细胞TNF-α的表达,从而抑制支气管扩张症气道炎症反应,这可能是其疗效机理之一。Objective To explore the role of TNF - α in the pathogenesis of bronchiectasis and the mechanism of therapeutical effiect of Zhikuoning Mixture on bronchiectasis. Methods In vitro cultured Human Bronchial Epithelial Cells（ HBE ） were divided into groups and stimulated by sputa from bronchiectaric patients. The expression of TNF - α in HBE and the effect of Zhikuoning Mixture on it were assessed. Results The expression of TNF - α in HBE was activated by sputa from bronchiectatic patients and Zhikuoning Mixture might suppress the expression. Conclusion The expression of TNF - α in HBE plays an important role in the pathogenesis of bronchiectasis ; Zhikuoning Mixture can suppress the TNF - st expression in HBE so as to improve airway inflammarion in bronchiectasis. This may be one of mechanisms of therapeutic effect of Zhikuoning Mixture on bronchiectasis.

关 键 词：支扩宁合剂 支气管扩张症 支气管上皮细胞 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]