毛泡桐叶中乌索酸的提取分离及反相高效液相色谱分析  

Preparation and RP-HPLC Analysis of Ursolic Acid from Paulownia tomentosa(Thunb.) Steud Leaves

作　　者：易艳萍[1] 邹盛勤[2] 谢晚彬[1] 陈武[1] 

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000 [2]江西省天然药物活性成分研究重点实验室,江西宜春336000

出　　处：《时珍国医国药》2008年第4期799-800,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家“863”计划重点资助项目(No.2002AA2Z3217)

摘　　要：目的提取鉴定毛泡桐叶中的乌索酸,并建立其含量测定方法。方法采用“醇提凝析法”新工艺分离制备乌索酸,IR,MS,NMR鉴定其结构。建立反相高效液相色谱测定其含量的方法,色谱柱为Krom asilC18柱,流动相为乙腈-甲醇-水-磷酸（体积比168∶682∶150∶1.68）,流速0.8 m l/m in,检测波长210 nm,柱温25℃。结果乌索酸在0.92-16.56μg范围内线性关系良好（r=0.999 6）。样品乌索酸含量为99.61%,得率为30.5%。结论“醇提凝析法”分离制备乌索酸工艺先进,实用可行,为工业试验生产乌索酸提供了技术条件;RP-HPLC法测定乌索酸含量准确、精密度高、重复性好、线性范围宽,可作为控制乌索酸质量的方法。Objective：To extract and identify ursolic acid from Paulownia tomentosa （Thunb.） Steud leaves and to establish a determination method of ursolic acid. Methods：Ursolic acid was extracted from Paulownia tomentosa（ Thunb. ） Steud leaves by adopting a new method of ethanol extraction and agglutination separation, and its structure was identified by IR, MS and NMR. A determination method of ursolic acid in Paulownia tomentosa （ Thunb. ） Steud leaves by reversed - phase high performance liquid chromtograph was established. The chromatographic column （ KromasilC18, 4.6 mm x 250 mm, 5μm ）, acetonitrile - methanol -water- phosphoric acid mobile phase（ 168：682：150：1.68, V/V） with 0.8 ml/min flow rate, the detected wavelength （210 nm） and the column temperature （25℃） were adopted. Results：Ursolic acid showed good linear relationship in the range of 0. 92 - 16.56μg（ r = 0. 999 6 ）. The average recovery rate was 99.82% （ RSD was 0.75% ）. The content was 99.61% and the gain rate was 3.05‰. Conclusion： The method of ethanol extraction and agglutination separation to extract ursolic acid is ad- vanced, rational, practical and feasible, and it provides technology condition for industrial production of ursolic acid. RP - HPLC is simple and accurate, it has good repeatability and wider linear range, and it can be used to evaluate the quality of ursolic acid.

关 键 词：毛泡桐叶 乌索酸 结构表征 反相高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]