中药壁虎抗肿瘤作用的实验研究  

Experimental Study on the Anti-tumor Action of Gecko

作　　者：刘菲[1] 王建刚[1] 席守民[1] 马晓娟[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003

出　　处：《时珍国医国药》2008年第4期957-959,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨中药壁虎抗肿瘤作用及其作用机制。方法建立移植瘤小鼠S180肉瘤模型,将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组,分别给于生理盐水灌胃1次/d,CTX(100 mg/kg)腹腔注射1次,壁虎高、中、低组(13.5,9,4.5 g/kg),每天灌胃1次。14 d后,称取荷瘤小鼠瘤重、胸腺重、脾脏重,计算抑瘤率、胸腺指数、脾脏指数,观察腹腔巨噬细胞吞噬鸡血红细胞实验指标;用SABC免疫组化法检测血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的蛋白表达,TUNEL方法检测细胞凋亡率。结果中药壁虎可抑制小鼠肉瘤S180生长,高、中、低组抑瘤率可达49.8%,52.8%,43.1%;对免疫器官无影响;可降低肿瘤组织VEGF,bFGF蛋白的表达,诱导肿瘤细胞凋亡。结论中药壁虎有抗肿瘤活性,其抗肿瘤的部分作用可能通过诱导肿瘤细胞的凋亡及抑制肿瘤血管的生成而实现。Objective To study the anti - tumor action of Gecko and its mechanism. Methods The transplanted tumor model of the mouse S180 sarcoma was established. Fifty mice were randomly divided into five groups, the control group, the CTX group, the Gecko group（ high dose, middle dose, low dose）. They were treated respectively with oral administration of saline, and intraperitoheal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day. After 14 days ,the anti - tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal maerophage which phagocytose chicken red blood cells. The protein express of vascular endothelin growth factor（VEGF） and basic fibroblast growth factor（bFGF） were detected by SABC immunohistochemistry. The cell apoptotic rate was detected by TUNEL method. Results Chinese medicine Gecko could obviously inhibit S180 sarcoma of mice,the inhibiting rate was 49.8% ,52.8% ,43.1% ,it had no impact on immune organ. It could reduce VEGF and bFGF protein expression in tumor tissue and induce tumor cell apoptosis. Conclusion Chinese medicine Gecko has anti - tumor activity and its mechanism might be related to induction of tumor cell apoptosis and the down - regulation of protein expression of VEGF and bFGF.

关 键 词：中药壁虎 小鼠肉瘤S180 血管内皮生长因子 碱性成纤维细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]