高效液相色谱法测定新疆一枝蒿中一枝蒿酮酸的含量  

Determination of Rupestonic Acid in Artemisia Rupestris L.Growing in Xinjiang by HPLC

作　　者：马成[1] 杨敏[1] 张烜[1] 堵年生[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054

出　　处：《时珍国医国药》2008年第4期991-992,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立新疆一枝蒿中一枝蒿酮酸含量测定的方法。方法采用高效液相色谱法（HPLC）,SupelcosilTMLC18色谱柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.4%磷酸水溶液（50∶50）;流速0.8 ml.min-1,柱温30℃,检测波长243nm。结果一枝蒿酮酸和其他组分可达基线分离,线性范围0.042 4-0.212 0μg（r=0.999 6）,平均回收率为96.4%,方法精密度RSD=2.5%（n=3）。结论该法操作简便快速、准确灵敏,分离效果好,可为新疆一枝蒿质量控制提供方法依据。Objective To establish an HPLC method for determining rupestonie acid in Artemisia rupestris L. growing in Xinjiang. Methods A Supelcosil^TM LC18 column （4.6 mm × 250 mm,5 μm） and mobile phase consisted of methanol -0.4% phosphoric acid solution （50：50） were used at the column temperature of 30℃. The flow rate was 0.8 ml · min^-1 and detection wavelength was 243 nm. Results The quantity of rupestonic acid in the range of 0.042 4 -0. 212 0μg versus peak area was linear （r = 0.999 6）. The average recovery was 96.4 % , and method precision was 2.8% （RSD =2.8% , n = 6）. Conclusion The method is simple, quick, accurate, high sensitive and suitable for quality control of Artemisia rupestris L. growing in Xinjiang.

关 键 词：一枝蒿 一枝蒿酮酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]