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作 者:赵杨[1] 靳凤云[2] 贺祝英[2] 廖薇[2] 梁光义[2]
机构地区:[1]四川大学华西药学院,四川成都610041 [2]贵阳中医学院,贵州贵阳550002
出 处:《时珍国医国药》2008年第5期1036-1038,共3页Lishizhen Medicine and Materia Medica Research
基 金:国家自然科学基金(No.30460154)
摘 要:目的测定华盖散传统汤剂与颗粒汤剂中苦杏仁苷的含量。方法采用高效液相色谱(HPLC)法。选用Agilent XDB-C18柱,流动相为以甲醇-水(23∶77),检测波长为215nm波长。结果苦杏仁苷的含量在传统汤剂与颗粒汤剂中有显著性差异,颗粒汤剂中平均含量大于传统汤剂中平均含量。结论该测定方法简便、准确,重复性良好,方中其它成分对测定无干扰。Objective To determine the Laetrile in the traditional decoction and kernel decoction of Huagai San. Methods HPLC was used. An Agilent XDB- C18 column was used, the mobile phase was methanol- water (23:77 ), the detection wavelength was 215nm. Results The content of Laetrile in traditional decoction and kernel decoction had obvious discrepancies, the average content in kernel decoction was more than that in traditional decoction. Conclusion The method is simple, convenient, exact and reproducible, the other ingredients do not have interferences for the determination.
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