高效液相色谱法测定复方三七缓释片中有效成分的含量  

Determination of Notoginsenoside R_1,Ginsenoside Rg_1,Ginsenoside Re and Ginsenoside Rb_1 in Compound Sanqi Sustained-release Tablets by HPLC

作　　者：刘波[1] 聂阳[1] 沈嘉茵[1] 谢凯[1] 吴燕红[1] 朱盛山[1] 

机构地区：[1]广东药学院中药开发研究所,广东广州510006

出　　处：《时珍国医国药》2008年第5期1048-1049,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672670);国家科技支撑计划课题任务书基金(No.2006BAI09B08-02)

摘　　要：目的建立复方三七缓释片中有效成分的高效液相测定方法。方法采用Agilent C18柱（250 mm×4.6 mm,5μm）;以乙腈-0.05%磷酸水溶液（乙腈0-12 min：22%;12-60 min：22%-40%）为流动相梯度洗脱,流速为1 ml·min^-1,检测波长为203 nm。结果三七皂苷R1和人参皂苷Rg1,Re,Rb1的线性范围分别为0.373-3.73μg（r=0.9997）,0.937-9.37μg（r=0.9991）,0.144-1.44μg（r=0.9991）,0.893-8.93μg（r=0.9990）;平均加样回收率（n=6）分别为99.12%,98.86%,99.25%,98.63%,RSD分别为1.31%,1.57%,1.75%,1.68%。结论该测定方法简便可行、重复性好,可用于复方三七缓释片中有效成分的含量测定。Objective To establish the method of determining effective components in Compound Sanqi Sustained - release Tablets by HPLC. Methods The contents of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were simultaneously determined by HPLC conditions as follows ：the column was Agilent C18 （250 mm ×4.6 mm, 5μm） ,the mobile phase was CH3CN -0.05% H3PO4 （CH3CN 0 - 12 min：22% ;12 -60 min：22% -40% ）, the flow rate was 1.0 ml · min^-1 , the detection wavelength was 203 nm. Results The linear range of notoginsenoside R1 , ginsenoside Rg1 , ginsenoside Re and ginsenoside Rb1 , respectively, were 0.373 -3.73μg（r = 0.999 7） ,0.937 -9.37 p,g（r = 0. 999 1 ）, 0. 144 - 1.44p, g（r =0. 999 1 ） ,0. 893 - 8.93 μg（ r = 0. 999 0 ） ; the average recoveries （ n = 4） were 99. 12% , 98.86% , 99.25 % and 98.63% respectively, and RSD were 1.31% ,1.57% ,1.75% and 1.68%. Conclusion The results indicate that the HPLC method is simple, accurate, highly selective and reproducible, thus it can be used for quality control of Compound Sanqi Sustained - release Tablets.

关 键 词：三七 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 三七皂苷R1 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]