中药清除羟自由基实验的改良  

The Experimental Improvement of Chinese Herbs Scavenging Hydroxyl Radical

作　　者：余庆皋[1] 刘捷频[1] 吴梅青[1] 

机构地区：[1]湘潭职业技术学院,湖南湘潭411102

出　　处：《时珍国医国药》2008年第5期1107-1108,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的对邻二氮菲-Fe2+氧化法进行改良,使其在用于自由基清除剂筛选时更为有效和更为敏感。方法在不同的pH环境、不同的保温时间及不同的测定波长条件下进行对比实验。结果邻二氮菲-Fe2+络合物在pH4.5的环境下及510 nm的测定波长时有较大的吸光度,30 min的保温时间使各管有较大并较为稳定的吸光度。结论通过对邻二氮菲-Fe2+氧化法的实验改良能提高该方法筛选自由基清除剂的灵敏度。Objective The improvement can be carried out to the oxidation of phenathroline - Fe^2＋ in order to be effective and more sensitive in screening the free radical scavenger. Methods Making experiments by comparing the conditions of different pH, heat preservations and various wavelengh. Results The complex of phenathroline -Fe^2＋ had great absorbance with 510 nm wavelength in the environment of pH4.5, and the absorbance was larger and more stable after the 30 min heat preservation. Conclusion The improvement can increase the sensibility for sereening the free radical scavenger.

关 键 词：自由基清除剂 邻二氮菲 羟自由基 

分 类 号：R283[医药卫生—中药学]