1份生产用中药蛇粗毒的DNA分子鉴定  

DNA Molecular Identification of One Snake Crude Venom Used for Production

作　　者：陈念[1] 赵树进[2] 韩丽萍[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640 [2]中国人民解放军广州军区广州总医院,广东广州510010

出　　处：《时珍国医国药》2008年第7期1578-1580,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中国人民解放军全军医药卫生科研基金(No.2006166125);广东省自然科学基金(No.06019716)

摘　　要：目的利用PCR技术鉴定干燥蛇粗毒的来源物种，并对基因组DNA的提取效果及提取过程中需注意的若干问题进行分析和讨论，同时还对若干针对不同长度目的基因扩增引物的使用效果进行验证。方法从1份已保存了7年的生产用干燥蛇粗毒中提取总DNA，并以之为模板进行PCR扩增、测序和序列分析。结果使用该方法可从蛇粗毒中提取得到微量的总DNA，并成功扩增得到0．45～1．18kh不同长度范围的目的片段；测序结果提交NCBI，并与GenBank中的同源序列进行BLAST比对。结论该蛇粗毒样品来自眼镜蛇，且极可能由多个不同的亚种或单倍型所组成。该技术有可能推广用于对动物粗毒的来源进行准确和直接地鉴定。Objective To identify dried snake venom by PCR. Methods To extract DNA and subsequent sequencing of the mitochondrial genes from a snake venom that had been preserved for nearly 7 years. Results Minimal genomic DNA could be extracted from the crude venom by this method, and from which different length of target regions that ranged within 0.45 - 1.18 kb were amplified successfully. All the sequences were submitted to NCBI and compared against their homologous sequences in the GenBank database. Conclusion Sequence alignment analyses approved that Naja naja was its most probable original specie, and this sample could be consisted by some different subspecies or haplotypes. This approach offers a straight forward method for direct and accurate identification of animal crude venoms.

关 键 词：蛇粗毒 分子鉴定 线粒体基因 DNA测序 

分 类 号：R282.73[医药卫生—中药学]