四川椭圆叶花锚愈伤组织的诱导  

Inducement of the Callus of Halenia elliptica D.Don in Sichuan Province

作　　者：覃筱燕[1] 杨林[1] 邵丽勉[1] 刘盛林[1] 

机构地区：[1]中央民族大学生命与环境科学院,北京100081

出　　处：《时珍国医国药》2008年第7期1588-1589,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30600849);国家民委基金资助项目(No.05ZY08);中央民族大学少数民族类医药"985"资助项目

摘　　要：目的探讨不同培养条件及不同的外殖体对椭圆叶花锚愈伤组织诱导的影响。方法以椭圆叶花锚种子培养的无菌苗的茎和新鲜植株的茎段为外殖体,MS为基本培养基,分别以6-BA和NAA两种植物激素为变量进行实验研究。结果MS培养基+1.0mg/L6-BA+1.0mg/L NAA对诱导椭圆叶花锚愈伤组织的形成效果最佳;高浓度的6-BA配合较高浓度的NAA诱导的愈伤组织致密,出愈率高。结论不同培养条件对椭圆叶花锚愈伤组织的诱导有不同的影响,其中以无菌苗的茎段为外殖体易形成愈伤组织。Objective To research the effects of different culture and different exophyte on the callus of Halenia elliptica D. Don. Methods With the stem of sterile seedling and fresh plants as exophyte, MS as a basic medium, 6 - BA and NAA hormone were adopted as the experimental variables. Results MS ＋ 1.0 mg / L 6 - BA ＋ 1.0 mg / L NAA induced the best effect of callus ; higher concentrations of 6 - BA with high concentrations of NAA made callus densification and higher rate. Conclusion Different plant hormones and their different concentration ratios have different effects on the callus of Halenia elliptica D. Don and adventitious buds.

关 键 词：外殖体 愈伤组织 椭圆叶花锚 

分 类 号：S567[农业科学—中草药栽培]