知母皂苷元对HEK293sw细胞APP和BACE1的影响  

Effects of active component of Zhimu on APP and BACE1 in HEK293sw cells

作　　者：张瑞[1] 王子玫[1] 夏宗勤[1] 胡雅儿[1] 

机构地区：[1]上海交通大学基础医学院细胞调控研究室,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第7期827-830,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市高校选拔培养优秀青年教师科研专项基金~~

摘　　要：目的探讨知母活性成分知母皂苷元(ZMS)对HEK293sw细胞淀粉样前体蛋白(APP)和β-位点APP切割酶1(BACE1)的影响。方法常规培养HEK293sw细胞,加入不同浓度ZMS预处理24 h后,换为无血清培养,继续处理48 h后观察ZMS的作用。Western blotting和RT-PCR技术测定APP和主要β-分泌酶BACE1的表达;荧光分析法测定BACE1活力。结果有效终浓度1μmol/L和10μmol/L的ZMS对BACE1的mRNA和蛋白表达均有显著降低作用,但对APP的mRNA和蛋白表达无明显影响。荧光分析结果显示,10μmol/L ZMS能显著降低BACE1活力。结论ZMS显著降低HEK293sw细胞主要β-分泌酶BACE1的表达及活力,而对APP的表达无明显影响。Objective To investigate the effects of ZMS, the active component of Zhimu, on amyloid precursor protein （APP） and β-site APP cleaving enzyme 1 （ BACE1 ） in HEK293sw cells. Methods HEK293sw cells were pretreated with different concentrations of ZMS in routine culture medium for 24 h, and then serum-free medium with ZMS was used for further culture for 48 h. Cells were examined for expression of APP and β-secretase BACE1 with Western blotting and RT- PCR, and were then examined for BACE1 activity with fluorescence method. Results 1 μmol/L and 10 μmol/L ZMS significantly decreased the expression of BACE1 mRNA and protein, while had no effect on the expression of APP mRNA and protein. It was indicated by fluorescence analysis that 10 μmol/L ZMS significantly decreased the β-secretase activity. Conclusion ZMS significantly decreases the activity and expression of β-secretase BACE1, while has no effect on the expression of APP in HEK293sw cells.

关 键 词：知母皂苷元 HEK293 sw细胞 Β-分泌酶 淀粉样前体蛋白 

分 类 号：R285.5[医药卫生—中药学] R966[医药卫生—中医学]