RIG-I基因剔除小鼠表型的初步分析  

Preliminary phenotype analysis of RIG-Ⅰ knockout mice

作　　者：孙岳平[1] 张丽君[2] 张梅[3] 金月娥[2] 柳子星[2] 张洪信[2] 陆顺元[2] 孔辉[2] 王铸钢[2] 

机构地区：[1]上海交通大学医学院细胞生物学教研室,上海200025 [2]上海交通大学医学院医学遗传学教研室,上海200025 [3]上海交通大学医学院病理学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第7期836-840,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的通过对视黄酸诱导基因Ⅰ(RIG-I基因)剔除小鼠表型的分析,在整体动物水平揭示RIG-Ⅰ的生物学功能。方法Northern blotting和半定量RT-PCR检测小鼠组织中RIG-I基因的表达;对小鼠的表型分析包括体质量的测量、外周血细胞分类计数、代谢指标测定及病理组织学检查。结果RIG-Ⅰ在小鼠多种组织中广泛表达,但表达水平存在一定差异。RIG-Ⅰ基因剔除后,小鼠未见明显发育异常;无明显预期的粒系分化受阻表现;但RIG-Ⅰ基因剔除小鼠外周血相中的粒/淋比明显倒置,且对条件性致病菌易感。结论RIG-Ⅰ在小鼠抗细菌免疫过程中具有重要作用。Objective To explore the biological functions of retinoic acid-inducible gene-Ⅰ （RIG-Ⅰ） in vivo through phenotype analysis of RIG-Ⅰ knockout mice. Methods The gene expression of RIG- Ⅰ in various tissues of mice was examined with Northern blotting and semi-quantitative RT-PCR. The phenotypes observed included body weight measurement, differential count of peripheral blood cells, metabolic parameters measurement and histopathologic examination. Results RIG-Ⅰ expressed in various tissues of mice with different levels. No gross developmental abnormalities and expected maturation arrest in granulocytic differentiation were observed in RIG-Ⅰ knockout mice. However, RIG-Ⅰ knockout mice exhibited an unexpected increase in the ratios of neutrophiles to lymphocytes in peripheral blood and increased susceptibility to bacteria infection. Conclusion RIG-Ⅰ may play an important role in immune regulation in mice.

关 键 词：视黄酸诱导基因Ⅰ 细菌感染 基因剔除小鼠 

分 类 号：Q789[生物学—分子生物学]