金属蛋白酶9在隐球菌性脑膜炎小鼠模型脑组织中的表达  

Expression of MMP-9 in brain tissue of mouse cryptococcal meningitis model

作　　者：徐赤宇[1] 温海[1] 朱红梅[1] 

机构地区：[1]第二军医大学长征医院皮肤科

出　　处：《第二军医大学学报》2008年第7期778-780,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30471566,30500441);大连市科研技术项目[大卫科发(2007)73号]~~

摘　　要：目的:观察基质金属蛋白酶-9(MMP-9)在鼠脑组织中的表达并探讨其意义。方法:雌性C57BL/6小鼠行股静脉插管后实验各组分别注入新生隐球菌菌悬液以及菌悬液加抑肽酶,8h后应用免疫组织化学技术检测MMP-9在小鼠脑组织中的表达情况;同时观测小鼠脑组织匀浆后菌落计数(CFU),并与免疫组织化学结果进行对照分析。结果:注入新生隐球菌菌悬液小鼠脑组织中的MMP-9显著升高,与正常对照组比较,有显著统计学差异(P<0.01);菌悬液加抑肽酶小鼠脑组织中MMP-9与正常对照组比较无明显统计学差异(P>0.05);MMP-9的染色强度与小鼠脑组织中的菌体负荷量正相关。结论:隐球菌性脑膜炎小鼠脑组织中存在MMP-9过表达,MMP-9可能导致血脑屏障开放程度提高。Objective： To explore the expression of MMP-9 in mouse brain tissue and its significance. Methods： Cryptococcosis suspension alone （fungal suspension group） or combined with aprotinin （aprotinin group） was injected in to female C57 BL/6 mice via femoral vein cannulation. The expression of MMP-9 was examined immunohistochemically 8 h later. Meanwhile,the mouse brain tissue homogenate colony count （CFU） was observed and the results were compared with that of the immunohistochemical examination. Results： The expression of MMP-9 was markedly increased in the fungal suspension group compared with the normal control group （P〈0.01） ; the expression in the aprotinin group was not significantly different from that of the normal control group （P〈0.05）. The amount of cryptococcosis in the brain tissue was positively correlated with expression of MMP-9. Conclusion： MMP-9 is overexpressed in the brain of mice with Cryptococcal meningitis; MMP-9 might lead to increased permeability of blood brain barrier.

关 键 词：隐球菌性脑膜炎 基质金属蛋白酶-9 抑肽酶 免疫组织化学 

分 类 号：R37-33[医药卫生—病原生物学]