复方丹参合剂对长期脂肪乳剂灌胃诱发的大鼠非酒精性脂肪肝病的影响  

Effect of a compound Danshen preparation in preventing long-term gastric lipid emulsion administration-induced nonalcoholic steatohepatitis in rats

作　　者：张志平[1] 尤婷婷[2] 邹丽宜[2] 吴铁[2] 吴怡[2] 崔燎[2] 

机构地区：[1]广东医学院组胚教研室,广东湛江524023 [2]广东医学院药理教研室,广东湛江524023

出　　处：《南方医科大学学报》2008年第7期1109-1113,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30672470)~~

摘　　要：目的用长期脂肪乳剂灌胃的方法建立大鼠高脂血症动物模型,探讨长期脂肪乳剂灌胃可否诱发大鼠非酒精性脂肪肝病,观察其肝脏病理学改变,探讨复方丹参合剂(DSC)对长期脂肪乳剂灌胃造成的大鼠高脂血症及非酒精性脂肪肝病是否有防治作用。方法取清洁级3m龄未交配SD大鼠27只,随机分成3组,1组为普通饲料对照组(普通饲料喂养,蒸馏水5ml/kg·d灌胃共20周),2组为脂肪乳剂组(脂肪乳剂5ml/kg·d灌胃连续20周),3组DSC组(上午脂肪乳剂灌胃5ml/kg·d,下午予DSC5.0g生药/kg·d灌胃,连续20周)。实验结束时剪鼠尾采末梢血测血糖(BG),大鼠心脏取血处死,分离血清测定谷丙转氨酶(ALT)、谷草转氨酶(AST)活力及总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-c)含量;肝组织称重,石蜡包埋制片进行组织学观察。结果与普通饲料组相比,脂肪乳剂组大鼠BG、TC、LDL-c和动脉粥样硬化指数(AI)均明显升高(P<0.01),HDL-c降低(P<0.01),TG无明显差异;脂肪乳剂组大鼠肝湿重和肝指数升高(P<0.01),血清ALT、AST明显升高(P<0.01),肝脏切片出现肝细胞脂肪变、水样变及坏死和炎症细胞浸润等病理表现;与脂肪乳剂组比较,大鼠DSC组BG、AI降低(P<0.01),但还没达到正常组的水平,HDL-c明显升高(P<0.01),肝湿重和肝指数、ALT、AST降低(P<0.05),但还没达到普通饲料组水平,肝脏切片显示肝细胞脂肪变、水样变及坏死和炎症细胞浸润等病理表现均有所减轻。结论脂肪乳剂长期灌胃可引起大鼠出现TC升高、HDL-c降低为特征的脂质代谢紊乱及非酒精性脂肪肝病,复方丹参合剂可提高长期脂肪乳剂灌胃的大鼠血清HDL-c,对长期脂肪乳剂灌胃引起的非酒精性脂肪肝病有一定的防治作用。Objective To investigate the preventive effects of a compound Danshen preparation （DSC） on long-term gastric lipid emulsion administration-induced nonalcoholic steatohepatitis in rats. Methods Twenty-seven 3-month-old SD rats were randomized equally into 3 groups and subjected to daily intragastric administration for 20 weeks of distilled water （control）, lipid emulsion at 5 ml/kg （model group）, and lipid emulsion plus DSC at 5.0 g/kg （DSC treatment group）. After blood glucose （BG） determination, the rats were sacrificed for measurement of serum TC, TG, HDL-c, AST, and ALT, and the liver was weighed and pathologically examined. Results Compared with the control group, the rats in the model group showed significantly increased BG, TC, LDL-c, arteriosclerosis index （AI）, AST, ALT, liver weight, and liver index （P〈0.01） and decreased HDL-c （P〈0.01）, while TG remained unchanged. Fatty degeneration, hydropic degeneration and necrosis with inflammatory cell infiltration were observed in the liver of the rats in the model group. Compared with the model group, the rats in DSC groups showed decreased BG, AI （P〈0.01）, liver weight, liver index, AST, and ALT （P〈0.05） and increased HDL-c, with milder pathological changes in the liver. Conclusions Long-term gastric perfusion of lipid emulsion causes lipid metabolic disorder and nonalcoholic fatty liver disease in rats characterized by increased TC and decreased HDL-c. DSC can significantly increase HDL-c and provide partial protection of the liver against the damages by the lipid emulsion.

关 键 词：脂肪乳剂 复方丹参合剂 非酒精性脂肪肝炎 肝脏组织病理学 大鼠 

分 类 号：R285.5[医药卫生—中药学]