陕南安康地区大竹园镇砷中毒患者血清PICP、PINP与COL1α1序列检测  

Detection of serum PICP and PINP and COL1α1 DNA sequence analysis in patients with arsenic poisoning in Dazhuyuan Town, Ankang area in southern Shaanxi Province

作　　者：王芝芳[1] 郭雄[1] 白广禄[2] 王立新[1] 雷艳霞[1] 

机构地区：[1]西安交通大学医学院公共卫生系,西安交通大学环境与疾病相关基因教育部重点实验室,西安陕西710061 [2]陕西省地方病防治研究所,西安陕西710003

出　　处：《南方医科大学学报》2008年第7期1141-1144,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30371252);陕西省国际合作重点项目(2005KW-13)~~

摘　　要：目的研究陕南安康地区大竹园镇高砷高氟高硒地区的砷皮肤损伤患者体内I型胶原代谢状况。方法采用病例对照研究,选取安康大竹园镇燃高砷高氟高硒石煤区29例砷皮肤损伤患者为病例组,西安市非砷中毒区30例为对照组。用石墨炉原子吸收光谱法测定砷和硒含量,氟离子选择性电极法测定氟含量,ELISA检测血清中I型前胶原羧基前肽(PICP)和I型前胶原氨基前肽(PINP)的含量,高保真PCR扩增-美国ABI3100测序仪作正反向直接测序检测I型胶原α1多肽链(COL1α1)碱基突变。结果在偏相关分析中控制其他影响因素后,PICP与血清氟呈弱负相关(r=-0.4,P≤0.05),PINP与发氟呈中度负相关(r=-0.5,P<0.05),与发硒呈中度正相关(r=0.5,P<0.05)。但血清PICP、PINP与COL1α1序列在病例组和对照组间无统计学显著性差异(P>0.05)。结论发氟和发硒是影响PINP代谢的主要影响因素,且发氟升高可使PINP降低而发硒升高可使PINP增高。但在陕南安康地区大竹园镇砷、氟和硒三元素联合性增高地区的砷皮肤损伤患者体内的PICP、PINP含量和I型胶原α1基因序列却无显著性改变。提示在该地区,发硒增高引起PINP的升高可能抑制了由发氟升高引起PINP的降低。Objective To investigate type Ⅰ collagen metabolism in patients with arsenic poisoning in Dazhuyuan town, Ankang area in southern Shaanxi province known for high-level exposure to arsenic （As）, fluoride （F） and selenium （Se）. Methods This case-control study involved 29 patients with arsenic poisoning from Dazhuyuan, a town in Ankang area of Shaanxi province affected by coal-bome endemic arsenicosis. Thirty individuals without arsenic poisoning from Chang＇an county of Xi＇an city were included as the control group. The serum concentrations of As and Se were determined using a graphite furnace atomic absorption spectrophotometer, and the levels of F detected with a fluoride ion-selective electrode. Enzyme-linked immunosorbent assay （ELISA） was used for assessing serum contents of PICP and PiNP, and the sequence of COL1α1 gene was analyzed with ABI sequence analyzer. Results The contents of PICP and PINP were similiar between the two groups, which had also identical COL1α1 DNA sequences. After controlling for all the other factors, an inverse correlation was noted between PICP and serum F contents （r=-0.4, P〈0.05） and between PINP and hair F content （r =-0.5, P〈0.05）, but a positive correlation was found between PINP and hair Se content （r=0.5, P〈0.05）. Conclusion Hair F and Se levels are the major factors affecting PINP synthesis, and increased hair F level is associated with elevated PINP content, whereas hair Se level is inversely correlated to PINP content. But in the patients with arsenic poisoning from Dazhuyuan town, the PICP, PINP contents and COL1α1 DNA sequence show no significant alterations, suggesting that hair Se increment abolishes the effect of hair F increment in inducing the decrease in PINP content.

关 键 词：氟 砷 硒 燃煤型砷中毒 Ⅰ型胶原 

分 类 号：R114[医药卫生—卫生毒理学] R127[医药卫生—公共卫生与预防医学]