霜天蛾昆虫变应原的变应原性及免疫原性分析  

Analysis of the allergenicity and immunogenicity of Psilogramma menephron allergen

作　　者：孙秀珍[1] 刘昀[1] 周玎[1] 李维[1] 冯向莉[1] 徐晶[1] 

机构地区：[1]西安交通大学医学院第二附属医院呼吸内科,陕西西安7100041

出　　处：《南方医科大学学报》2008年第7期1161-1164,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30271160)~~

摘　　要：目的分析霜天蛾主要变应原的变应原性和免疫原性,为进一步制备霜天蛾标准化变应原疫苗和基因工程重组霜天蛾昆虫变应原奠定基础。方法应用不连续聚丙烯酰胺凝胶电泳对霜天蛾变应原蛋白成分进行分离,对9例霜天蛾过敏患者血清分别进行IgE、IgG免疫印记分析,并对比分析IgE、IgG反应变应原成分。结果霜天蛾提取液经SDS-PAGE染色后显示出20余种蛋白质成分,相对分子质量12000-128000。免疫印迹分析结果显示具有变应原性的蛋白相对分子质量及反应率分别为:74000(88.9%),66000(22.2%),49000(22.2%),36000(77.8%),25000(33.3%),具有免疫原性的蛋白相对分子质量及反应率分别为:79000(33.3%),74000(66.7%),66000(22.2%),49000(22.2%),36000(44.4%),25000(55.6%)。结论霜天蛾变应原中具有较强变应原活性的蛋白质相对分子质量为74000和36000,具有较强免疫原活性的蛋白质相对分子质量为74000和25000的蛋白质,是霜天蛾过敏患者特异性过敏诊断和治疗的主要成分。Objective To analyze the allergenicity and immunogenicity ofPsilogramma menephron allergen so as to provide the basis for preparing recombinant and standardized allergen vaccines of Psilgramma menephorn. Methods The extracts of Psilgramma menephorn were analyzed by SDS-PAGE, and the allergenicity and immurtogenic ty of the extracts were tested with 9 sera from allergic patients by means of immunoblotting. Results More than 20 allergen proteins were separated from the extract ofPsilgramma menephorn by SDS-PAGE, with the relative molecular weight ranging from 12000 to 128000. The relative molecular weight of the allergenic proteins were 74 000 （88.9%）, 66 000 （22.2%）, 49 000 （22.2%）, 36 000 （77.8%）, or 25 000 （33.3%）, and those of the immunogenic proteins were 79 000 （33.3%）, 74 000 （66.7%）, 66000 （22.2%）, 49 000 （22.2%）, 36 000 （44.4%）, or 25 000 （55.6%）. Conclusion The relative molecular weight of the major allergenic proteins of Psilgramma menephorn are 74 000 and 36000, and 74 000 and 25 000 for the major immunogenic proteins. These proteins constitute the major allergenic components for diagnosis and specific treatment ofPsilgramma menephorn allergy.

关 键 词：霜天蛾 变应原 昆虫 变应原性 免疫原性 

分 类 号：R392.8[医药卫生—免疫学]