噬菌体生物扩增法早期诊断女性生殖器结核的临床研究  

Early diagnosis of female genital tuberculosis by phage amplified biological assay

作　　者：刘宝瑛[1] 李艳[1] 郑磊[2] 钟梅[2] 余艳红[2] 熊梦舟[2] 

机构地区：[1]广州市胸科医院妇科,广东广州510095 [2]南方医科大学南方医院妇产科,广东广州510515

出　　处：《南方医科大学学报》2008年第7期1313-1314,共2页Journal of Southern Medical University

基　　金：广东省科技厅资助项目(73108)

摘　　要：目的探讨应用噬菌体生物扩增法(噬菌体法)检测白带中的结核分枝杆菌在早期诊断女性生殖器结核的临床价值。方法选择2005年1月-2007年9月间我院收治的86例病理确诊为女性生殖器结核的病人。所有病例在治疗前应用噬菌体法检测白带中的分枝杆菌,同时白带分别送检荧光定量PCR、涂片和培养检测分枝杆菌,对照检查结果。结果86例患者中噬菌体法45例阳性,阳性率52.3%,特异性为100%,PCR的阳性率为23.3%,假阳性率为20%;培养法5例阳性,阳性率5.8%;涂片法全部阴性。噬菌体法明显高于涂片和培养法(P<0.01)。结论噬菌体生物扩增法在诊断女性生殖器结核中具有较高的敏感性和特异性,为早期诊断女性生殖器结核提供重要的临床价值。Objective To establish an early diagnostic method for detecting female genital tuberculosis. Methods Eighty-six women with genital tuberculosis during January 2005-September 2007 were examined by phage amplified biological assay, and the results were compared with those from leucorrhea culture, smear and PCR. Results Forty-five patients were tuberculosis positive with 100% of specificity identified by phage amplified biological assay. Twenty patients were tuberculosis positive by＇PCR. Five patients were culture-positive tuberculosis and no case had smear-positive tuberculosis. Conclusion Phage amplified biologically assay is sensitive and specific, which could be used for the early diagnosis of female genital tuberculosis.

关 键 词：女性生殖器结核 分枝杆菌噬菌体 分枝杆菌 

分 类 号：R446.5[医药卫生—诊断学] R527[医药卫生—临床医学]