全反式维甲酸对人白血病细胞系U937细胞生长的影响及其机制分析  

Growth inhibition of human leukemia cell line U937 by all-trans retinoic acid and its mechanism

作　　者：赵玥铭[1] 王燏婵[1] 陆牡丹[1] 沈爱国[1] 张冬梅[1] 陆建新[2] 程纯[1] 

机构地区：[1]南通大学微生物与免疫学教研室,226001 [2]南通大学附属医院

出　　处：《中华血液学杂志》2008年第7期464-467,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（30300099）;江苏省自然科学基金（BK2003035）;江苏省高校自然科学研究项目（03KJB180109、04KJB320114）;江苏省科技指导性计划（BS2004526）

摘　　要：目的研究全反式维甲酸（ATRA）作用于人白血病细胞系U937细胞后，对细胞生长的影响及可能的机制。方法收集1 μmoL／L ATRA作用后的U937细胞，利用流式细胞术检测细胞周期分布，Western blot法检测细胞周期相关蛋白（cyclinA、p16、p21、p27及p27相关分子skp2）表达水平的变化，采用免疫沉淀方法检测U937细胞中p27与Skp2的结合情况。结果流式细胞术分析显示，在1 μm0L／L ATRA作用72h后，72％的U937细胞被阻滞在G0／G1期。Western blot分析显示，ATRA能诱导cyclinA、Skp2表达下凋，p21、p27表达上调。免疫沉淀法检测结果显示在U937细胞中p27与Skp2存在相互结合的情况。结论ATRA可能主要通过诱导p27表达上调引起U937细胞生长阻滞，其过程是由p27相关分子Skp2所介导的。Objective To study the effect of all-trans retinoic acid （ATRA） on U937 cell growth and its mechanism. Methods Cell cycle was detected by flow cytometry （ FCM）, expressions of cell cycle associated protein and the p27 related protein were detected by Western blot. The binding of P27 and Skp2 was detected by immunoprecipitation. Results FCM displayed that ATRA could inhibit the proliferation of U937 cells. At 72 h on 1 μmol/L ATRA treatment, 72% of the cells were arrested at G0/G1 phase. Western blot displayed that ATRA could decrease the expression of cyclinA, up-regulate the expression of p21 and p27, and down-regulate the expression of p27 related proteins Skp2. p27 could bind with Skp2 in U937 cells as detected by immunoprecipitation. Conclusion ATRA may arrest the proliferation of U937 cells through the reduction of Skp2 expression, and finally the induction of the accumulation of p27.

关 键 词：维甲酸 U937细胞 P27蛋白 SKP2蛋白 

分 类 号：R686[医药卫生—骨科学]