针刺对川芎嗪防治豚鼠庆大霉素中毒性耳聋靶向作用的影响  

Effect of acupuncture on target action of Ligustrazine in treatment of toxic deafness induced by Gentamicin in guinea pigs

作　　者：郑华平[1] 王枫[1] 徐泉珍[2] 徐飞[1] 张国军[1] 王鹏[2] 王永华[1] 刘金洪[1] 范炳华[2] 

机构地区：[1]浙江中医药大学耳聋康复研究所,杭州350003 [2]浙江中医药大学针灸推拿学院,杭州350003

出　　处：《中国针灸》2008年第7期515-518,共4页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:30472230

摘　　要：目的:探讨治疗庆大霉素中毒性耳聋的有效方法及作用机制。方法:将豚鼠随机分为正常对照组(A组)、模型组(B组)、川芎嗪组(C组)、针刺组(D组)和针刺加川芎嗪组(E组)。C、D、E组分别给予单纯川芎嗪、单纯针刺(穴取"听宫""翳风""外关")和针刺联合川芎嗪治疗。10d后检测听性脑干诱发电位(ABR)Ⅲ波反应阈值、Corti器部位细胞凋亡及Bax、Bcl-2蛋白表达。结果:E组ABR阈值低于C组(P<0.05),Corti器部位细胞凋亡、Bax蛋白表达及Bax/Bcl-2比值均低于C组和D组,Bcl-2蛋白表达有所增加。结论:针刺"听宫""翳风"及"外关"穴对川芎嗪有一定的靶向协同作用,可提高治疗庆大霉素中毒性耳聋的疗效。其作用机制可能与抑制细胞凋亡、下调Bax蛋白表达及上调Bcl-2蛋白表达有关。Objective To explore an effective method for treating Gentamicin-induced deafness and the mechanism. Methods Guinea pigs were randomly divided into 5 groups： normal control group （group A）, model group （group B）, Ligustrazine group （group C）, acupuncture group （group D） and Ligustrazine plus acupuncture group （group E）. The group C, D and E were treated respectively by simple Ligustrazine, simple acupuncture at ＂Tinggong＂ （SI 19）, ＂Yifeng＂ （TE 17）, and ＂Waiguan＂ （TE 5）, and Ligustrazine plus acupuncture. Ten days later, the auditory brainstem response （ABR） thresholds for the wave Ⅲ, apoptosis and expressions of Bcl-2 and Bax proteins in the organ of Corti of the guinea pig were detected. Results In the group E, the ABR threshold was significantly lower than that in the group C （P〈0. 05）, and apoptotic cells, the expression of Bax protein and the ratio of Bax/Bcl-2 were lower than those in the group C and D in the organ of Corti, and Bcl-2 protein expression was increased. Conclusion Acupuncture at ＂Tinggong＂ （SI 19）, ＂Yifeng＂ （TE 17）, and ＂Waiguan＂ （TE 5） has a certain target-synergistic action on Ligustrazine and can increase therapeutic effect of Ligustrazine on Gentamicin-induced deafness, which are possible related with the inhibition of apoptosis, down-regulation of Bax expression and up-regulation of Bcl-2 expression.

关 键 词：聋/针灸疗法 庆大霉素类/中毒 川芎嗪/治疗应用 药物协同作用 豚鼠 

分 类 号：R246.81[医药卫生—针灸推拿学]